Conjugation of a low affinity nerve growth factor receptor (p75NTR) antibody to saporin has produced a cytotoxin that eliminates the CBF neurons, while sparing neighboring neurons that express GAD, calbindin and parvalbumin. mu p75-SAP eliminates cells expressing p75NTR in mouse. Permanent and selective removal of cholinergic forebrain neurons makes an important animal model for the study of behavior, neuronal loss (e.g. Alzheimer’s disease), plasticity of other systems in response to loss, replacement therapy, and drug effects and dependence.
mu p75-SAP is a chemical conjugate of the affinity-purified rabbit polyclonal antibody p75NTR and the ribosome-inactivating protein, saporin. It specifically eliminates p75NTR-positive cells in mouse.