We are highlighting our ZAP antibody internalization kits, as well as our line of secondary antibody saporin conjugates. These products are given the moniker “ZAP”in place of saporin.
The ZAP conjugates produced by ATS consist of a variety of secondary antibodies that allow a large number of targeting agents to be screened quickly and cost-efficiently for specificity, functional binding, internalization, and EC50 determination.
The conjugates are constructed using either species-specific secondary antibodies, or streptavidin (for use with biotinylated Targeting Agents), and they are chemically attached to Saporin, a potent plant ribosome-inactivating protein.
Use of a ZAP conjugate eliminates the time-consuming and expensive step of conjugating each targeting-agent-candidate to the payload. The ZAP conjugate can simply be reacted with a targeting agent in a single step and added to cells in culture conditions.
Once the reacted conjugate has been administered, the Targeting Agent seeks out and binds its receptor and takes the saporin payload inside the cells of interest, where it is released within the cytosol to inactivate the ribosomes. Cells that don’t express the target cell surface marker don’t bind or internalize the ZAP-targeting agent complex, and won’t be affected. Saporin has no binding chain, and no means of getting into cells on its own.
