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pHast Conjugates are one of our fastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day. The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates.
Also available: Streptavidin-pHast (PH-03). Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast). This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.
| Target | Recommended Products | ||
|---|---|---|---|
| alpaca/llama IgG (VHH domain) | Alpaca-pHast (PH-05) | ||
| chicken IgY | Chick-pHast (PH-06) | ||
| goat IgG | Goat-pHast (PH-07) | ||
| guinea pig IgG | gPIG-pHast (PH-08) | ||
| human IgG | Hum-pHast (PH-09) | Fab-pHast human (PH-01) | FabFc-pHast human (PH-10) |
| human IgM | Hug-M-pHast (PH-11) | FabFc-pHast Hug-M (PH-04) | |
| mouse IgG | Mab-pHast (PH-12) | Fab-pHast mouse (PH-02) | FabFc-pHast mouse (PH-13) |
| mouse IgM | Anti-M-pHast (PH-14) | ||
| rabbit IgG | Rab-pHast (PH-15) | Fab-pHast rabbit (PH-16) | |
| rat IgG | Rat-pHast (PH-17) | Fab-pHast rat (PH-18) | FabFc-pHast rat (PH-19) |
 made with bivalent antibodies that recognize the whole IgG |  made with monovalent (Fab) antibodies that recognize the whole IgG, without bivalent capping |  made with monovalent (Fab) antibodies that recognize Fc region only |