pHast Conjugates
pHast Conjugates are one of our pHastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day.
How It Works
The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. pHast conjugates are fluorescent plate reader compatible and can be used to illuminate your lead antibody candidates in a 96-well plate format.
Also Available:
Streptavidin-pHast (PH-03) 
Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast).
All of our pHast Conjugates are available with these fluorescent dyes.
- The pHast RED has an excitation wavelength of 532 nm with an emission maxima at 560 nm.
- The pHast FAR-RED has an excitation wavelength of 643 nm with an emission maxima at 660 nm.
- The pHast GREEN version has an excitation wavelength of 453 nm with an emission maxima at 522 nm.
- The pHast BLUE has an excitation wavelength of 362 nm with an emission maxima at 452 nm.
| Target | Color | Recommended Products made with whole IgG | made with monovalent (Fab) | made with monovalent Fc region (FabFc) |
|---|---|---|---|---|
| alpaca/llama IgG (VHH domain) | | Alpaca-pHast (VHH) (PH-05) | Fab-pHast Alpaca (VHH) (PH-21) | |
| alpaca/llama IgG | | Alpaca-pHast (IgG) (PH-20) | ||
| chicken IgY | | Chick-pHast (PH-06) | ||
| goat IgG | | Goat-pHast (PH-07) | ||
| guinea pig IgG | | gPIG-pHast (PH-08) | ||
| human IgG | | Hum-pHast (PH-09) | Fab-pHast human (PH-01) | FabFc-pHast human (PH-10) |
| human IgM | | Hug-M-pHast (PH-11) | FabFc-pHast Hug-M (PH-04) | |
| mouse IgG | | Mab-pHast (PH-12) | Fab-pHast mouse (PH-02) | FabFc-pHast mouse (PH-13) |
| mouse IgM | | Anti-M-pHast (PH-14) | ||
| rabbit IgG | | Rab-pHast (PH-15) | Fab-pHast rabbit (PH-16) | |
| rat IgG | | Rat-pHast (PH-17) | Fab-pHast rat (PH-18) | FabFc-pHast rat (PH-19) |
 made with bivalent antibodies that recognize the whole IgG |  made with monovalent (Fab) antibodies that recognize the whole IgG, without bivalent capping |  made with monovalent (Fab) antibodies that recognize Fc region only |
View a complete product listing here.