Advanced Targeting Systems’ technology – Molecular Surgery – is a modification of one of the most widely used techniques in the neurosciences: lesioning of a region by surgical means and observation of the effect.
Saporin has no way of entering a cell on its own, except by bulk-phase endocytosis. To be effective, Saporin is bound to a Targeting Agent. A Targeting Agent binds to the cell surface and is internalized. Examples include: antibody, ligand, cytokine, growth factor, aptamer, etc. When the conjugate is internalized it escapes the endosomal compartment and inactivates the ribosomes. This shuts down protein synthesis and results in cell death.