Author name: Kristen Hartman

Key Features. Real Advantages. Internalization data is only useful if you can trust what you’re seeing. Because pHast Conjugates activate specifically in acidic intracellular compartments, they help distinguish internalized material from surface-bound signal and reduce the background that can complicate interpretation with traditional assays. The result is a clearer, more biologically relevant readout of internalization

One Platform. Multiple Workflows. pHast Conjugates are designed to support a range of internalization workflows across different targeting materials and assay platforms. Researchers can use Fab-pHast to evaluate antibody fragments or Streptavidin-pHast to assess biotinylated targeting materials, including antibodies, peptides, and other compatible formats. The assay is also adaptable across multiple readout platforms, including microscopy, flow cytometry, and high-throughput screening.

True internalization. pHast Conjugates were built to answer a simple but critical question: did your targeting material actually internalize? The pHast reporter is designed to remain silent at neutral pH and activate only after internalization into acidic intracellular compartments such as endosomes and lysosomes. That means you can distinguish true internalization from surface-bound signal and

Less Guessing. More Knowing. Determining whether a targeting material truly internalizes can be one of the biggest challenges in early-stage evaluation. Binding alone does not guarantee internalization, and traditional fluorescent assays often create more uncertainty than clarity. Surface-bound signal, background fluorescence, and complex wash steps can all make it difficult to know which candidates are