FabFc-ZAP uses your primary rat IgG antibody to target and eliminate cells that recognize your primary antibody. The secondary antibody used in the Fc-specific Fab-ZAP will react only with the Fc portion of the IgG heavy chain. The FabFc-ZAP products can be used in B-cell research with the confidence that cell death is primary antibody-mediated. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
FabFc-ZAP rat is a chemical conjugate of goat anti-rat monovalent antibody and the ribosome-inactivating protein, saporin. It uses your rat primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
FabFc-ZAP rat is available individually (Cat. #IT-88) or as a kit (Cat. #KIT-88) which includes FabFc-ZAP rat, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.