FabFc-ZAP human [IT-65, KIT-65]

Name: FabFc-ZAP human [IT-65, KIT-65]
SKU: IT-65
Availability: InStock

a tool to “piggyback” onto YOUR antibody via goat anti-human monovalent antibody; targeting cells that recognize YOUR human antibody, eliminated via saporin

Size

Clear

SKU: IT-65 Category: ZAP Conjugates Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug, Kit (1 ab) w/controls & developing reagents, tests 1 ab, Kit (4 ab) w/controls & developing reagents, tests 4 abs, Kit (10 ab) w/controls & developing reagents, tests 10 abs | Antibody Type: affinity-purified, Polyclonal | Host: goat | Reactivity: human | Conjugate: saporin | Usage: eliminates cells, screen antibodies |

FabFc-ZAP uses your primary human IgG antibody to target and eliminate cells that recognize your primary antibody. The secondary antibody used in the Fc-specific Fab-ZAP will react only with the Fc portion of the IgG heavy chain. The FabFc-ZAP products can be used in B-cell research with the confidence that cell death is primary antibody-mediated. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

FabFc-ZAP human is a chemical conjugate of goat anti-human monovalent antibody and the ribosome-inactivating protein, saporin. It uses your human primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

FabFc-ZAP human is available individually (Cat. #IT-65) or as a kit (Cat. #KIT-65) which includes FabFc-ZAP human, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: antibody, efficacy, immunotoxin, screening, internalization, saporin, Fab-ZAP, FabFc-ZAP, monovalent Fc region, secondary conjugate, primary antibody, anti-human monovalent antibody

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View Data Sheet

Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin.

Kohls MD, Lappi DA (2000) Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin. BioTechniques 28(1):162-165. doi: 10.2144/00281pf01

Summary: Immunotoxins are useful tools for elimination of specific cell populations in vitro and in vivo for research and therapeutic applications. One of the factors limiting the use of immunotoxins is the selection of an appropriate antibody. Advanced Targeting Systems has created a reagent that allows researchers to select antibodies with the desired characteristics before an immuntoxin is made, purified, and assayed. Using a goat anti-murine IgG coupled to the ribosome-inactivating protein saporin, researchers can screen hundreds of antibodies in a time and cost-effective manner.

Related Products: Mab-ZAP (Cat. #IT-04), Rab-ZAP (Cat. #IT-05), Hum-ZAP (Cat. #IT-22), Rat-ZAP (Cat. #IT-26), Anti-M-ZAP (Cat. #IT-30), Goat-ZAP (Cat. #IT-36)

Streptavidin-saporin: Converting biotinylated materials into targeted toxins

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181

Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.

Read the full article.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

Read complete article.

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