zap-conjugates

Mab-ZAP binds to Fc portion of mouse IgG

Q: Does Mab-ZAP (Cat. #IT-04) bind to the FC portion of mouse IgG? A: The antibody used to create our...

FabFc-ZAP cross-reaction with another species

Q: Can your FabFc-ZAP human (Cat# IT-65) bind to the Fc portion of another species, such as mouse IgG? It...

Dosage of Fab-ZAP for antibody concentration

Q: Is the dosage of Fab-ZAP always enough for any level of antibody concentration? A: The 4.5 nM dosage of...

Recommended ratio between Fab-ZAP dosage and antibody concentration

Q: Is there a recommended ratio between Fab-ZAP dosage and antibody concentration? A: A recommended good starting point is 4.5...

Adjust number of replications

Q: Each concentration is suggested to perform 6 replications, can it be adjusted more or less in practice? A: Yes,...

Detecting the targeted antibody in supernatant

Q: Can Fab-ZAP detect the targeted antibody still in supernatant? A: As long as there is nothing in the supernatant...

DMSO% for peptide and Streptavidin-ZAP

Q: Instead of performing the reaction between our biotinylated peptide and Streptavidin-ZAP at the initially provided concentration of Strep-ZAP (20...

ZAP Internalization Kit Concentrations

Q: We have your ZAP internalization kit and I have a question regarding the concentrations used in the cytotoxicity assay....

Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected...

Light-controlled elimination of PD-L1+ cells

Wong JJW, Selbo PK (2021) Light-controlled elimination of PD-L1+ cells. J Photochem Photobiol B 225:112355. doi: 10.1016/j.jphotobiol.2021.112355 Objective: To investigate...

Autosomal recessive osteopetrosis: mechanisms and treatments

Penna S, Villa A, Capo V (2021) Autosomal recessive osteopetrosis: mechanisms and treatments. Dis Model Mech 14(5):dmm048940. doi: 10.1242/dmm.048940 Summary:...

Activated factor X targeted stored in platelets as an effective gene therapy strategy for both hemophilia A and B

Wang D, Shao X, Wang Q, Pan X, Dai Y, Yao S, Yin T, Wang Z, Zhu J, Xi X,...

Bone marrow-derived myeloid progenitors in the leptomeninges of adult mice

Koeniger T, Bell L, Mifka A, Enders M, Hautmann V, Mekala SR, Kirchner P, Ekici AB, Schulz C, Wörsdörfer P,...

Identification of prostaglandin F2 receptor negative regulator (PTGFRN) as an internalizable target in cancer cells for antibody-drug conjugate development

Marquez J, Dong J, Dong C, Tian C, Serrero G (2021) Identification of prostaglandin F2 receptor negative regulator (PTGFRN) as...

Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency.

Castiello MC, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, Draghici E, Corsino C, Bortolomai I, Sereni L,...

Pseudomonas Exotoxin A based toxins targeting epidermal growth factor receptor for the treatment of prostate cancer

Fischer A, Wolf I, Fuchs H, Masilamani AP, Wolf P (2020) Pseudomonas Exotoxin A based toxins targeting epidermal growth factor...

Cellular uptake of vitamin B12: Role and fate of TCblR/CD320, the transcobalamin receptor.

Gick GG, Arora K, Sequeira JM, Nakayama Y, Lai SC, Quadros EV (2020) Cellular uptake of vitamin B12: Role and...

The EphA2 and cancer connection: potential for immune-based interventions

London M, Gallo E (2020) The EphA2 and cancer connection: potential for immune-based interventions. Mol Biol Rep 47(10):8037-8048. doi: 10.1007/s11033-020-05767-y...

ALPPL2 is a highly specific and targetable tumor cell surface antigen

Su Y, Zhang X, Bidlingmaier S, Behrens CR, Lee NK, Liu B (2020) ALPPL2 is a highly specific and targetable...

Nongenotoxic antibody-drug conjugate conditioning enables safe and effective platelet gene therapy of hemophilia A mice.

Gao C, Schroeder JA, Xue F, Jing W, Cai Y, Scheck A, Subramaniam S, Rao S, Weiler H, Czechowicz A,...