Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.
Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.
Streptavidin-ZAP is available individually (Cat. #IT-27) and in multiple kit variations.
|Biotinylated Antibody||Biotinylated Peptide||Other Biotinylated Material|
|your material||primary antibody species: goat, human, mouse, rabbit, or rat||peptide||any|
|in vivo or in vitro||
in vivo kit (KIT-27-A) includes Streptavidin-ZAP and BIgG-SAP for your species
in vitro kit (KIT-27-Z) includes Streptavidin-ZAP, BIgG-SAP for your species, and Saporin plus reagents for developing a cytotoxicity assay (saporin, PMS, XTT)
in vivo kit (KIT-27-B) includes Streptavidin-ZAP and Blank-Streptavidin-SAP
in vitro kit (KIT-27-ZB) includes Streptavidin-ZAP, Blank-Streptavidin-SAP, and Saporin plus reagents for developing a cytotoxicity assay (saporin, PMS, XTT)
|kit (KIT-27-S) includes Streptavidin-ZAP and saporin|
|converts biotinylated materials into targeted toxins||✔||✔||✔|
|evaluate the ability of your reagent to internalize upon binding to its receptor||✔||✔||✔|
|kit contents||• Streptavidin-ZAP
• BIgG-SAP for your species
|• Streptavidin-ZAP||• Streptavidin-ZAP|