a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin
Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.
Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.
Streptavidin-ZAP is available individually (Cat. #IT-27) and in multiple kit variations. See below for kit options.
These kits convert biotinylated materials into targeted toxins and will allow you to evaluate the ability of your reagent to internalize upon binding to its receptor.
keywords: Streptavidin, Avidin, Biotin, screening, ADC, Antibody Drug Conjugate, in vivo, saporin, streptavidinylated, Streptavidin-SAP, Streptavidin-ZAP, Strep-ZAP, Strep-SAP
Reviews
sisi.zheng –
Technical support was extremely valuable in ensuring we ordered the right products that suited our research needs. The website also contains multiple useful protocols and online calculators to determine how much of the streptavidin-ZAP to incubate with our biotinylated antibody. Order arrived promptly and well packaged.
annie.gagnon –
Streptavidin-ZAP is routinely tested by cytotoxicity assay. It is formatted in PBS solution without preservative, and it is sterile. There is no need to do further purifications. So, it is very convenient for downstream cell killing experiments both in vitro and in vivo. This product is also time-saving. Just incubate your biotinylated material with Streptavidin-ZAP for 15min at room temperature, then it’s ready for use. In addition, biotin and streptavidin has the strongest known noncovalent biological interaction. It is essentially nonreversible. Don’t worry about the dissociation. Saporin protein can effectively target and kill the cell with your specific biotinylated material once they are internalized by the cell.
ahmed.jalal –
Worked seamlessly. Technical support was excellent.
Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.
Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184 PMID: 35324681
Summary: Saporin is a ribosome-inactivating protein that can cause inhibition of protein synthesis and causes cell death when delivered inside a cell. Development of commercial Saporin results in a technology termed ‘molecular surgery’, with Saporin as the scalpel. Its low toxicity (it has no efficient method of cell entry) and sturdy structure make Saporin a safe and simple molecule for many purposes. The most popular applications use experimental molecules that deliver Saporin via an add-on targeting molecule. These add-ons come in several forms: peptides, protein ligands, antibodies, even DNA fragments that mimic cell-binding ligands. Cells that do not express the targeted cell surface marker will not be affected. This review will highlight some newer efforts and discuss significant and unexpected impacts on science that molecular surgery has yielded over the last almost four decades. There are remarkable changes in fields such as the Neurosciences with models for Alzheimer’s Disease and epilepsy, and game-changing effects in the study of pain and itch. Many other uses are also discussed to record the wide-reaching impact of Saporin in research and drug development.
Objective: To further investigate and develop the utility of CD45-SAP and CD117-SAP, in combination with immunosuppression, in murine transplant models using i.v. administration in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).
Summary: CD45-SAP or CD117-SAP in combination with immunosuppressants (30Fll and post-transplant Cytoxan) enabled >85% peripheral donor chimerism at 12 weeks post-transplantation. CD45-SAP and CD117-SAP were more effective at conditioning versus 2Gy TBI or pretransplant Cytoxan.
Usage: CD45-SAP (1.9 mg/kg, iv) and CD117-SAP (1mg/kg, iv) in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).
Van Hentenryck M, Li Z, Murphy PM, Czechowicz A (2022) Antibody-based preparative regimens for cell, tissue and organ transplantation. (eds. 162). OBM Transplantation 6(3):162. doi: 10.21926/obm.transplant.2203162
Objective: Provide a review of progress in the use of antibodies to support cell and tissue transplantation with a particular focus on induction of donor-specific tolerance for solid organ transplantation.
Summary: Antibody-based conditioning to prepare the recipient is a promising approach towards achieving transplant tolerance in both hematopoietic and solid organ transplant settings.
Usage: To enhance HSC depletion while avoiding bystander toxicity (neutropenia, lymphopenia, and thrombocytopenia) caused by CD45-radioimmunotherapy, Palchaudhuri et al. developed a saporin-based CD45 (CD45-SAP) immunotoxin using a biotinylated antibody and Streptavidin-ZAP.
Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods
Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.
sisi.zheng –
Technical support was extremely valuable in ensuring we ordered the right products that suited our research needs. The website also contains multiple useful protocols and online calculators to determine how much of the streptavidin-ZAP to incubate with our biotinylated antibody. Order arrived promptly and well packaged.
annie.gagnon –
Streptavidin-ZAP is routinely tested by cytotoxicity assay. It is formatted in PBS solution without preservative, and it is sterile. There is no need to do further purifications. So, it is very convenient for downstream cell killing experiments both in vitro and in vivo. This product is also time-saving. Just incubate your biotinylated material with Streptavidin-ZAP for 15min at room temperature, then it’s ready for use. In addition, biotin and streptavidin has the strongest known noncovalent biological interaction. It is essentially nonreversible. Don’t worry about the dissociation. Saporin protein can effectively target and kill the cell with your specific biotinylated material once they are internalized by the cell.
ahmed.jalal –
Worked seamlessly. Technical support was excellent.