Streptavidin-ZAP is available individually (Cat. #IT-27) and in multiple kit variations. See below for kit options.
Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.
Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.
These kits convert biotinylated materials into targeted toxins and will allow you to evaluate the ability of your reagent to internalize upon binding to its receptor.
| Target | Recommended Product in vivo | Recommended Product in vitro |
|---|---|---|
| biotinylated peptide | Streptavidin-ZAP Peptide Kit (KIT-27-B) | Biotin-Z Peptide Internalization Kit (KIT-27-ZB) |
| biotinylated antibody *choose host species of your material | Streptavidin-ZAP Antibody Kit (KIT-27-A) goat, human, mouse, rat, or rabbit | Biotin-Z Antibody Internalization Kit (KIT-27-Z) goat, human, mouse, rat, or rabbit |
| other biotinylated material | Streptavidin-ZAP Kit (KIT-27-S) | Streptavidin-ZAP Kit (KIT-27-S) |
Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.
Data Sheet | Related Products | References | Articles | Videos | Protocols
Streptavidin-ZAP Antibody Kit (KIT-27-A)
Streptavidin-ZAP Peptide Kit (KIT-27-B)
Biotin-Z Antibody Internalization Kit (KIT-27-Z)
Biotin-Z Peptide Internalization Kit (KIT-27-ZB)
Streptavidin-ZAP Kit (KIT-27-S)
FITC-Streptavidin-ZAP (Cat. #IT-85)
Streptavidin-pHast (Cat. #PH-03)
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Streptavidin-saporin: Converting biotinylated materials into targeted toxins
Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181
Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.
Related Products: Streptavidin-ZAP (Cat. #IT-27)
Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184
Palchaudhuri R, Hyzy SL, Proctor JL, Adams HL, Pearse BR, Sarma G, Aslanian S, Gillard G, Lamothe TL, Burenkova O, Brooks ML, Gabros AD, McDonagh CF, Boitano AE, Cooke MP (2018) Antibody drug conjugates targeted to CD45 or CD117 enable allogeneic hematopoietic stem cell transplantation in animal models. Blood 132:3324. doi: 10.1182/blood-2018-99-119432
Objective: To further investigate and develop the utility of CD45-SAP and CD117-SAP, in combination with immunosuppression, in murine transplant models using i.v. administration in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).
Summary: CD45-SAP or CD117-SAP in combination with immunosuppressants (30Fll and post-transplant Cytoxan) enabled >85% peripheral donor chimerism at 12 weeks post-transplantation. CD45-SAP and CD117-SAP were more effective at conditioning versus 2Gy TBI or pretransplant Cytoxan.
Usage: CD45-SAP (1.9 mg/kg, iv) and CD117-SAP (1mg/kg, iv) in an allogeneic minor mismatch transplant model (Balb/c donor into DBA/2 recipients).
Related Products: Streptavidin-ZAP (Cat. #IT-27)
Antibody-based preparative regimens for cell, tissue and organ transplantation
Van Hentenryck M, Li Z, Murphy PM, Czechowicz A (2022) Antibody-based preparative regimens for cell, tissue and organ transplantation. (eds. 162). OBM Transplantation 6(3):162. doi: 10.21926/obm.transplant.2203162
Objective: Provide a review of progress in the use of antibodies to support cell and tissue transplantation with a particular focus on induction of donor-specific tolerance for solid organ transplantation.
Summary: Antibody-based conditioning to prepare the recipient is a promising approach towards achieving transplant tolerance in both hematopoietic and solid organ transplant settings.
Usage: To enhance HSC depletion while avoiding bystander toxicity (neutropenia, lymphopenia, and thrombocytopenia) caused by CD45-radioimmunotherapy, Palchaudhuri et al. developed a saporin-based CD45 (CD45-SAP) immunotoxin using a biotinylated antibody and Streptavidin-ZAP.
Related Products: Streptavidin-ZAP (Cat. #IT-27)
See Also:
- Palchaudhuri R Featured Article: Targeted depletion of hematopoietic stem cells promises safer transplantation. Targeting Trends 17(3), 2016.
- Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.
Evaluate Potential Targeting Molecules.
Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods
Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.
Related Products: Streptavidin-ZAP (Cat. #IT-27)
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Streptavidin-ZAP Concentration
Equimolar Mixing of Strep-ZAP and Biotinylated Molecule
DMSO% for peptide and Streptavidin-ZAP
Streptavidin-ZAP concentration ratio
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Biotin-Z Internalization Kit Introduction
Biotin-Z Internalization Kit Tutorial
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Biotin-Z (Streptavidin-ZAP) literature
Preparing and Interpreting Cytotoxicity Data in vitro
Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity