Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.
Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.
Streptavidin-ZAP is available individually (Cat. #IT-27) and in multiple kit variations.
| Antibody Kit KIT-27-A | Peptide Kit KIT-27-B | Biotin-Z Kit KIT-27-Z | |
| your material | primary antibody species: goat, human, mouse, rabbit, or rat | peptide | any |
| internalization assay | in vivo | in vivo | in vivo |
| converts biotinylated materials into targeted toxins | ✔ | ✔ | ✔ |
| evaluate the ability of your reagent to internalize upon binding to its receptor | ✔ | ✔ | ✔ |
| kit contents | • Streptavidin-ZAP • BIgG-SAP for your species goat (Cat. #IT-81) | • Streptavidin-ZAP | • Streptavidin-ZAP • Reagents for developing a cytotoxicity assay |
