Streptavidin-ZAP [IT-27, KIT-27]

a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin

SKU: IT-27 Category: Conjugate: saporin | Usage: converts biotinylated materials, eliminates cells |
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Streptavidin-ZAP converts biotinylated materials into targeted toxins. Streptavidin is a tetrameric protein (molecular weight 53 kDa in its recombinant form), with each subunit able to bind a single biotin molecule. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The streptavidin used to make Streptavidin-ZAP contains no carbohydrate group and has a neutral isoelectric point, which therefore reduces the nonspecific binding as compared to avidin. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and reacted with streptavidin-labeled probes or other detection reagents for use in biological assays.

Streptavidin-ZAP is a chemical conjugate of streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins.

Streptavidin-ZAP is available individually (Cat. #IT-27) and in multiple kit variations.

Biotinylated Antibody Biotinylated Peptide Other Biotinylated Material
your material primary antibody species: goat, human, mouse, rabbit, or rat peptide any
in vivo or in vitro

in vivo kit (KIT-27-A) includes Streptavidin-ZAP and BIgG-SAP for your species

in vitro kit (KIT-27-Z) includes Streptavidin-ZAP,  BIgG-SAP for your species, and Saporin plus reagents for developing a cytotoxicity assay (saporin, PMS, XTT)

in vivo kit (KIT-27-B) includes Streptavidin-ZAP and Blank-Streptavidin-SAP

in vitro kit (KIT-27-ZB) includes Streptavidin-ZAP, Blank-Streptavidin-SAP, and Saporin plus reagents for developing a cytotoxicity assay (saporin, PMS, XTT)

kit  (KIT-27-S) includes Streptavidin-ZAP and saporin
converts biotinylated materials into targeted toxins
evaluate the ability of your reagent to internalize upon binding to its receptor
kit contents Streptavidin-ZAP

BIgG-SAP for your species

goat (Cat. #IT-81)
human (Cat. #IT-77),
mouse (Cat. #IT-74),
rabbit (Cat. #IT-75),
rat (Cat. #IT-73)

Streptavidin-ZAP

Blank-Streptavidin-SAP (Cat. #IT-27B)

Streptavidin-ZAP

Saporin (Cat. #PR-01)