MonoBiotin-ZAP [BT-ZAP]

$300.00

a tool to “piggyback” onto YOUR streptavidinylated material via biotin; targeting cells that recognize YOUR streptavidinylated material, eliminated via saporin

SKU: BT-ZAP Categories: , Conjugate: Biotin | Usage: eliminates cells |

Saporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha2-macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death. The bond between streptavidin and biotin is rapid and essentially non-reversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents. It is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand.

This conjugate recognizes streptavidin conjugates. MonoBiotin-ZAP (BT-ZAP) is a chemical conjugate of saporin labeled with biotin in a 1:1 chemically-determined average molar ratio. MonoBiotin-ZAP uses YOUR streptavidinylated targeting agent to target and eliminate cells.