Streptavidin-pHast is one of our fastest tools for quantitative testing of your biotinylated protein’s specificity, binding, and internalization, providing results in 1 day. Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast). This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.
This secondary conjugate recognizes YOUR biotinylated protein. Streptavidin-pHast is a conjugate of streptavidin and a pH-dependent fluorescent reporter. Approximately 17 micrograms of Streptavidin-pHast is enough to test 1 biotinylated protein (6 replicates at 8 different concentrations) with a starting concentration of 100 nM in a 96-well plate.