Streptavidin-pHast [PH-03]

a tool to test protein specificity, binding, and internalization with results in one (1) day

SKU: PH-03 Category: Conjugate: pH-dependent fluorescent reporter | Usage: screen antibodies |

Streptavidin-pHast is one of our fastest tools for quantitative testing of your biotinylated protein’s specificity, binding, and internalization, providing results in 1 day.  Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast).  This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.

This secondary conjugate recognizes YOUR biotinylated protein. Streptavidin-pHast is a conjugate of streptavidin and a pH-dependent fluorescent reporter.  Approximately 17 micrograms of Streptavidin-pHast is enough to test 1 biotinylated protein (6 replicates at 8 different concentrations) with a starting concentration of 100 nM in a 96-well plate.

See: Fab-pHast human (Cat. #PH-01), Fab-pHast mouse (Cat. #PH-02), Streptavidin-pHast (Cat. #PH-03)