FITC-Streptavidin-ZAP [IT-85]

a tool to “piggyback” onto YOUR biotinylated material via streptavidin; targeting cells that recognize YOUR biotinylated material, eliminated via saporin and fluorescence via fluorescein isothiocyanate (FITC)

SKU: IT-85 Categories: , Conjugate: FITC, streptavidin-saporin | Usage: converts biotinylated materials, eliminates cells |

There are a growing number of antibody and small molecule therapeutic candidates and this demands a quick and efficient technique to screen for biomarkers that internalize effectively upon binding. FITC-Streptavidin-ZAP (FITC-SA-ZAP) provides for the efficient determination of internalization of cell surface biomarkers upon binding of antibodies or peptides. The construct that makes this method effective was formed by crosslinking a fluorescent reporter, in this case fluorescein (FITC) and Streptavidin (SA) to the ribosome-inactivating protein, saporin (ZAP).

The conjugate used in screening potential targeting agents or cells is a mixture of a biotinylated targeting agent mixed in a 1:1 molar ratio with FITC-labeled Streptavidinylated saporin (FITC-SA-ZAP). The bond between Streptavidin and biotin is rapid and essentially nonreversible, unaffected by most extremes of pH, organic solvents, and denaturing reagents.  The method provides a definitive assay readout: fluorescence within 1 hour and cell death in 72 hours. This method is designed for rapid screening, in a quick and reproducible manner, for specificity and internalization in various cell types to explore suitability of targeting agents.

FITC-Streptavidin-ZAP is a chemical conjugate of fluorescein (FITC), streptavidin and the ribosome-inactivating protein, saporin. It converts biotinylated materials into targeted toxins and made fluorescent with FITC.

FITC-Streptavidin-ZAP is available individually (Cat. #IT-85) or unlabeled Streptavidin-ZAP (Cat. #IT-27).