FabFc-ZAP Hug-M uses your primary human monoclonal IgM antibody to target and eliminate cells that recognize your primary antibody. The secondary antibody used in FabFc-ZAP Hug-M will react only with the Fc portion of the IgM heavy chain. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
FabFc-ZAP Hug-M is a chemical conjugate of goat anti-human IgM antibody and the ribosome-inactivating protein, saporin. It uses your human primary IgM antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
FabFc-ZAP Hug-M is available individually (Cat. #IT-78) or as a kit (Cat. #KIT-78) which includes FabFc-ZAP Hug-M, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.
Other ZAP Conjugates:
keywords: antibody, efficacy, immunotoxin, screening, internalization, saporin, Fab-ZAP, FabFc-ZAP, monovalent Fc region, secondary conjugate, primary antibody, anti-human IgM