FabFc-pHast Hug-M [PH-04]

a tool to test antibody specificity, binding, and internalization with results in one (1) day

SKU: PH-04 Category: Antibody Type: affinity-purified, Polyclonal | Host: Goat | Reactivity: Human | Conjugate: pH-dependent fluorescent reporter | Usage: screen antibodies |

FabFc-pHast Hug-M is one of our fastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day. Fab-pHast Hug-M binds to your primary human IgM antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates.

FabFc-pHast Hug-M generates quantitative testing of the specific, binding, and internalization of your primary human antibody, with results in 1-day. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

See: Fab-pHast human (Cat. #PH-01), Fab-pHast mouse (Cat. #PH-02), Streptavidin-pHast (Cat. #PH-03)