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Fab-ZAP number of replications

Q: Each concentration is suggested to perform 6 replications, can it be adjusted more or less in practice?

A: Yes, the assay design is meant to be a thorough approach but can be adjusted by the user. We recommend 6 replications based on our 96-well plate template design. The concentration of Fab-ZAP is 4.5 nM in the suggested protocols.

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