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cytotoxicity-assay

Mab-ZAP binds to Fc portion of mouse IgG

Q: Does Mab-ZAP (Cat. #IT-04) bind to the FC portion of mouse IgG? A: The antibody used to create our...

FabFc-ZAP cross-reaction with another species

Q: Can your FabFc-ZAP human (Cat# IT-65) bind to the Fc portion of another species, such as mouse IgG? It...

Dosage of Fab-ZAP for antibody concentration

Q: Is the dosage of Fab-ZAP always enough for any level of antibody concentration? A: The 4.5 nM dosage of...

Recommended ratio between Fab-ZAP dosage and antibody concentration

Q: Is there a recommended ratio between Fab-ZAP dosage and antibody concentration? A: A recommended good starting point is 4.5...

Adjust number of replications

Q: Each concentration is suggested to perform 6 replications, can it be adjusted more or less in practice? A: Yes,...

Detecting the targeted antibody in supernatant

Q: Can Fab-ZAP detect the targeted antibody still in supernatant? A: As long as there is nothing in the supernatant...

ZAP Internalization Kit Concentrations

Q: We have your ZAP internalization kit and I have a question regarding the concentrations used in the cytotoxicity assay....

in vitro Cytotoxicity Assays

Q: For in vitro cytotoxicity assays, could you tell me: 1) whether you incubate primary with your Saporin secondary for...

Flow cytometry for AB-N07

Q: We have a publication in review and put this statement in the paper, “The mouse monoclonal antibody to the...

Cytotoxicity Assay Protocols

Q: We are setting up some experiments in rat where we’d use your 192-IgG-SAP (Cat. #IT-01) in cytotoxicity assays. How...

Assay Parameters for Mab-ZAP

Q: Using Mab-ZAP (Cat. #IT-04) in a cytotoxicity assay, I obtained a nice dose-response curve up to around 10-9 M...