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Dosage of Fab-ZAP for antibody concentration

Q: Is the dosage of Fab-ZAP always enough for any level of antibody concentration?

A: The 4.5 nM dosage of Fab-ZAP is the recommended concentration.  We do not typically see unspecific killing at 4.5 nM on most cell lines.  If the concentration of Fab-ZAP is increased, it may undergo bulk-phase endocytosis and kill cells indiscriminately.  A lower concentration of Fab-ZAP may lead to antibody competition, resulting in a lack of killing of cells at the highest concentration of antibody.

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