Hug-M-ZAP uses your primary human monoclonal IgM antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-human IgM) to “piggyback” onto YOUR human primary antibody. Hug-M-ZAP can be utilized for screening human IgM antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Hug-M-ZAP is a chemical conjugate of affinity-purified goat anti-human IgM and the ribosome-inactivating protein, saporin. It uses your primary human monoclonal IgM antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Hug-M-ZAP is available individually (Cat. #IT-43) or as a kit (Cat. #KIT-43) which includes Hug-M-ZAP, Saporin (Cat. #PR-01), Goat IgG-SAP (Cat. #IT-19) and reagents for developing a cytotoxicity assay.