Hum-ZAP uses your primary human monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified goat anti-human IgG) to “piggyback” onto YOUR human primary antibody. Hum-ZAP can be utilized for screening human IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Hum-ZAP is a chemical conjugate of affinity-purified goat anti-human IgG and the ribosome-inactivating protein, saporin. It uses your human primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Hum-ZAP is available individually (Cat. #IT-22) or as a kit (Cat. #KIT-22) which includes Hum-ZAP, Saporin (Cat. #PR-01), Goat IgG-SAP (Cat. #IT-19) and reagents for developing a cytotoxicity assay.
