Rat-ZAP uses your primary rat monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. The secondary conjugate uses the secondary antibody (affinity-purified goat anti-rat IgG) to “piggyback” onto YOUR rat primary antibody. Rat-ZAP can be utilized for screening rat IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Rat-ZAP is a chemical conjugate of affinity-purified goat anti-rat IgG and the ribosome-inactivating protein, saporin. It uses your rat primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Rat-ZAP is available individually (Cat. #IT-26) or as a kit (Cat. #KIT-26) which includes Rat-ZAP, Saporin (Cat. #PR-01), Goat IgG-SAP (Cat. #IT-19) and reagents for developing a cytotoxicity assay.
Other ZAP Conjugates:
Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.
keywords: Rat Antibody, Rat Primary, Anti-Rat IgG, Goat, Affinity-purified, Screening, ADC, Antibody Drug Conjugate, in vitro, secondary
Evaluate Potential Targeting Molecules.
Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods
Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.
Related Products: Streptavidin-ZAP (Cat. #IT-27)
Read the article.
browse all references for this product | back to top