Rat-ZAP uses your primary rat monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. The secondary conjugate uses the secondary antibody (affinity-purified goat anti-rat IgG) to “piggyback” onto YOUR rat primary antibody. Rat-ZAP can be utilized for screening rat IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Rat-ZAP is a chemical conjugate of affinity-purified goat anti-rat IgG and the ribosome-inactivating protein, saporin. It uses your rat primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Rat-ZAP is available individually (Cat. #IT-26) or as a kit (Cat. #KIT-26) which includes Rat-ZAP, Saporin (Cat. #PR-01), Goat IgG-SAP (Cat. #IT-19) and reagents for developing a cytotoxicity assay.
