Fab-ZAP uses your primary rat IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of rat IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Rat-ZAP in a cytotoxicity assay. Fab-ZAP can be utilized for screening rat IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Fab-ZAP rat is a chemical conjugate of goat anti-rat monovalent antibody and the ribosome-inactivating protein, saporin. It uses your rat primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Fab-ZAP rat is available individually (Cat. #IT-55) or as a kit (Cat. #KIT-55) which includes Fab-ZAP rat, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.
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