Fab-ZAP uses your primary mouse monoclonal IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of mouse IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Mab-ZAP in a cytotoxicity assay.
Fab-ZAP mouse is a chemical conjugate of goat anti-mouse monovalent antibody and the ribosome-inactivating protein, saporin. It uses your mouse primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Fab-ZAP mouse is available individually (Cat. #IT-48) or as a kit (Cat. #KIT-48) which includes Fab-ZAP mouse, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.