Fab-pHast mouse [PH-02]

a tool to test antibody specificity, binding, and internalization with results in one (1) day

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SKU: PH-02 Category: Product Size: 100 ug, 250 ug, 1000 ug | Antibody Type: affinity-purified, Polyclonal | Host: Goat | Reactivity: Mouse | Conjugate: pH-dependent fluorescent reporter | Usage: screen antibodies |

Description

Fab-pHast mouse is one of our fastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day. Fab-pHast mouse binds to your primary mouse antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates.

Fab-pHast mouse generates quantitative testing of the specific, binding, and internalization of your primary mouse antibody, with results in 1-day. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols

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