Fab-pHast mouse [PH-02]

a tool to test antibody specificity, binding, and internalization with results in one (1) day

SKU: PH-02 Category: Antibody Type: affinity-purified, Polyclonal | Host: Goat | Reactivity: Mouse | Conjugate: pH-dependent fluorescent reporter | Usage: screen antibodies |

Fab-pHast mouse is one of our fastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day.  Fab-pHast mouse binds to your primary mouse antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates.

Fab-pHast mouse generates quantitative testing of the specific, binding, and internalization of your primary mouse antibody, with results in 1-day.  This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.  Making a 1.5 ml “Fab-pHast spiked solution” will use about 43 micrograms of Fab-pHast.  This is assuming  a 500-nM spiked solution and performing an assay on a 96-well plate, which is the suggested amount on the protocol.  This is enough to test 1 antibody in sextuplicate (6 data points) at 8 different concentrations of antibody.

See: Fab-pHast human (Cat. #PH-01), Streptavidin-pHast (Cat. #PH-03), FabFc-pHast HugM (Cat. #PH-04)