Fab-ZAP rabbit [IT-57, KIT-57]

a tool to “piggyback” onto YOUR antibody via goat anti-rabbit monovalent antibody; targeting cells that recognize YOUR rabbit affinity-purified antibody, eliminated via saporin

SKU: IT-57 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug, Kit (1 ab) w/controls & developing reagents, tests 1 ab, Kit (4 ab) w/controls & developing reagents, tests 4 abs, Kit (10 ab) w/controls & developing reagents, tests 10 abs | Antibody Type: affinity-purified | Host: goat | Reactivity: rabbit | Conjugate: saporin | Usage: eliminates cells, screen antibodies |

Fab-ZAP uses your primary rabbit affinity-purified IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of rabbit IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Rab-ZAP in a cytotoxicity assay. Fab-ZAP can be utilized for screening rabbit IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.

Fab-ZAP rabbit is a chemical conjugate of goat anti-rabbit monovalent antibody and the ribosome-inactivating protein, saporin. It uses your rabbit affinity-purified antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.

Fab-ZAP rabbit is available individually (Cat. #IT-57) or as a kit (Cat. #KIT-57) which includes Fab-ZAP rabbitSaporin (Cat. #PR-01)Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.

Other ZAP Conjugates:

Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.

keywords: antibody, efficacy, immunotoxin, screening, internalization, saporin, Fab-ZAP, monovalent antibody, secondary conjugate, primary antibody, goat anti-rabbit monovalent antibody


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Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin.

Kohls MD, Lappi DA (2000) Mab-ZAP: A tool for evaluating antibody efficacy for use in an immunotoxin. BioTechniques 28(1):162-165. doi: 10.2144/00281pf01

Summary: Immunotoxins are useful tools for elimination of specific cell populations in vitro and in vivo for research and therapeutic applications. One of the factors limiting the use of immunotoxins is the selection of an appropriate antibody. Advanced Targeting Systems has created a reagent that allows researchers to select antibodies with the desired characteristics before an immuntoxin is made, purified, and assayed. Using a goat anti-murine IgG coupled to the ribosome-inactivating protein saporin, researchers can screen hundreds of antibodies in a time and cost-effective manner.

Related Products: Mab-ZAP (Cat. #IT-04), Rab-ZAP (Cat. #IT-05), Hum-ZAP (Cat. #IT-22), Rat-ZAP (Cat. #IT-26), Anti-M-ZAP (Cat. #IT-30), Goat-ZAP (Cat. #IT-36)

Streptavidin-saporin: Converting biotinylated materials into targeted toxins

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2023) Streptavidin-saporin: Converting biotinylated materials into targeted toxins. Toxins 15(3):181. doi: 10.3390/toxins15030181

Summary: This manuscript describes the myriad of ways Streptavidin-ZAP is used and how this technology supports the scientific process of ‘Molecular Surgery’ and progress in research and drug development. Insights from publications and research performed using Streptavidin-ZAP and its impact on academia and industry for research and drug development are presented.

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Related Products: Streptavidin-ZAP (Cat. #IT-27)

Evaluate Potential Targeting Molecules.

Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods

Summary: Targeted toxins -- targeting agents conjugated to saporin -- are widely used to eliminate specific cell populations both in vitro and in vivo. For these molecules to be effective, it is vital that the targeting component of the conjugate specifically binds the cells of interest. A secondary conjugate, Streptavidin-ZAP, has been created by attaching the toxin saporin to streptavidin. The user can combine primary biotinylated material with Streptavidin-ZAP to quickly and economically screen potential targeting molecules for internalization and specificity. Once the appropriate targeting molecule is identified, a direct conjugation with saporin can be performed.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

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Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom

Ancheta LR, Shramm PA, Bouajram R, Higgins D, Lappi DA (2022) Saporin as a commercial reagent: its uses and unexpected impacts in the biological sciences-tools from the plant kingdom. Toxins (Basel) 14(3):184. doi: 10.3390/toxins14030184

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Fab IgG-SAP (Cat. #IT-67)

Fab-ZAP human (Cat. #IT-51)

Fab-ZAP mouse (Cat. #IT-48)

Fab-ZAP rabbit (Cat. #IT-57)

Fab-ZAP rat (Cat. #IT-55)

Fab-pHast rabbit (Cat. #PH-16)

Saporin (Cat. #PR-01)

Rab-ZAP (Cat. #IT-05)

Targeting Tools: Fab-ZAPs

Targeting Tools: FabFc-ZAP – A new secondary conjugate

Choosing the Correct Secondary Conjugate

Detecting the targeted antibody in supernatant

Targeting B Cells

Targeting Tools: ZAP Antibody Internalization Kit

ZAP Antibody Internalization Kits

Fab-ZAP Final Concentration

Dosage of Fab-ZAP for antibody concentration

Recommended ratio between Fab-ZAP dosage and antibody concentration

Which secondary ZAP conjugate should I use?

Fab-ZAP number of replications

Higher concentration and less cytotoxicity

ZAP Antibody Internalization Kit Introduction

ZAP Antibody Internalization Kit Tutorial

ZAP Antibody Internalization Kit literature

Cytotoxicity Assay Protocol for ZAP Antibody Internalization Kits

Online Calculator: Preparing ZAP Antibody Kit Samples

Concentration Calculations: Convert molarity to mg/ml and mg/ml to molarity (PDF worksheet)

Preparing and Interpreting Cytotoxicity Data in vitro

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