Fab-ZAP uses your primary rabbit affinity-purified IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of rabbit IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Rab-ZAP in a cytotoxicity assay. Fab-ZAP can be utilized for screening rabbit IgG antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Fab-ZAP rabbit is a chemical conjugate of goat anti-rabbit monovalent antibody and the ribosome-inactivating protein, saporin. It uses your rabbit affinity-purified antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Fab-ZAP rabbit is available individually (Cat. #IT-57) or as a kit (Cat. #KIT-57) which includes Fab-ZAP rabbit, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.