Fab-ZAP uses your primary human IgG antibody to target and eliminate cells that recognize your primary antibody. Fab-ZAP is made with a monovalent secondary antibody eliminating the possibility of cap formation, while preserving all the qualities that make an effective in vitro diagnostic tool. The antibodies used are affinity-purified polyclonal antibodies against both the heavy and light chain of human IgG. The antibody used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). It also has an improved EC50 when directly compared to Hum-ZAP in a cytotoxicity assay.
Fab-ZAP human is a chemical conjugate of goat anti-human monovalent antibody and the ribosome-inactivating protein, saporin. It uses your human primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
![](https://atsbio.com/wp-content/uploads/2024/05/IT-51-cytotox-trastuzumab.jpg)
SK-BR-3 cells were plated at 1000 cells/90 μl/well and incubated overnight. Trastuzumab and Saporin dilutions were made in cell media and 10 μl was added to each well. The Trastuzumab was also diluted in cell media containing, at a final concentration, 4.5 nM/10 μl Fab-ZAP, and 10 μl was added to each well. The plates were incubated for 72 hours. The plates were developed using a solution of XTT/PMS and read at 450 nm. Cytotoxicity was analyzed by comparing well readings of the treated wells to those of the control wells, expressed as a percentage. The number of viable cells remaining on the day of development is measured via cell metabolism of a colorimetric molecule within the developing reagents. Analysis was performed using Prism software (GraphPad, San Diego).
Fab-ZAP human is available individually (Cat. #IT-51) or as a kit (Cat. #KIT-51) which includes Fab-ZAP human, Saporin (Cat. #PR-01), Fab IgG-SAP (Cat. #IT-67) and reagents for developing a cytotoxicity assay.
Other ZAP Conjugates:
Need another ZAP Conjugate for your target? Check here for other species & targets including in vivo and in vitro options.
keywords: antibody, efficacy, immunotoxin, screening, internalization, saporin, Fab-ZAP, monovalent antibody, secondary conjugate, primary antibody, goat anti-human monovalent antibody
Verified User –
I have tried your Fab-Zap assay here recently and your protocols are spot on. It worked great.
Verified User –
I had the pleasure of ordering the FAB-ZAP human and mouse kit from your company a few months ago. The results achieved with this kit have been truly outstanding and I appreciate the quality of your products.