pHast Conjugates are one of our fastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day. Alpaca-pHast binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates.
Other pHast Conjugates:
View a table of targets and recommended pHast conjugates for testing your primary antibody specificity.
Also available: Streptavidin-pHast (PH-03). Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast).