ATS scientists Leonardo Ancheta and Patrick Shramm walk through the internalization challenge, how pHast works, and why it can help accelerate candidate evaluation across a wide range of targeting materials and assay platforms.
In this short video, we break down the core pHast mechanism and show how the reporter activates only after internalization, helping researchers screen candidates with greater confidence and less ambiguity.
Whether you are screening early candidates, comparing targeting materials, or building a broader internalization workflow, pHast offers a flexible platform for generating rapid, quantitative internalization data.
pHast Conjugates help distinguish internalized material from surface-bound signal and reduce the background. The result is a clearer, more biologically relevant readout of internalization with less ambiguity and less assay complexity.
An introduction to our pHast product line: pH-sensitive fluorescent secondary conjugates designed to fluoresce when exposed to the environment inside of a cell.
