True internalization.

pHast Conjugates were built to answer a simple but critical question: did your targeting material actually internalize?

The pHast reporter is designed to remain silent at neutral pH and activate only after internalization into acidic intracellular compartments such as endosomes and lysosomes. That means you can distinguish true internalization from surface-bound signal and generate a more direct readout of cellular uptake.

In this short video, we break down the core pHast mechanism and show how the reporter activates only after internalization, helping researchers screen candidates with greater confidence and less ambiguity.

How pHast Works: 1. Targeting Material. Your targeting agent of interest. 2. Cell Binding. Binds specifically to the target on the cell surface. 3. Internalization & Activation. The bound conjugate is internalized via endocytosis and activated in acidic compartments. 4. Clear Internalization Data. A strong fluorescent signal indicates true internalization and delivers clear, confident date on your platform.