References

Polito L (2022) Antibody based delivery of toxins and other active molecules for cancer therapy. Biomedicines 10(2):267. doi: 10.3390/biomedicines10020267 PMID: 35203476

Summary: This introduction to studies collected in a special issue that confirmed the potential of immunotherapy for targeted therapy in different cancer models. In the near future, antibody-based therapeutic approaches could improve the outcomes of cancer patients, overcoming some difficulties associated with standard therapy.

Biomedicines, Volume 10, Issue 2 (February 2022) – 318 articles

Pinelli F, Saadati M, Zare EN, Makvandi P, Masi M, Sacchetti A, Rossi F (2023) A perspective on the applications of functionalized nanogels: promises and challenges. International Materials Reviews 68(1):1-25. doi: 10.1080/09506608.2022.2026864

Summary: A perspective on the applications of functionalized nanogels: their features such as large surface area, ability to hold molecules, flexibility in size and water-based formulations have ensured them great recognition as drug delivery systems with various in vivo applications which have confirmed their potential.

Konsman JP (2022) Cytokines in the Brain and Neuroinflammation: We Didn’t Starve the Fire!. Pharmaceuticals (Basel) 15(2):140. doi: 10.3390/ph15020140 PMID: 35215252

Summary: To test the role of IL-1 receptor brain macrophages, the work of Chaskiel et al. employed IL-1 coupled to the ribosome toxin, saporin, and administered this conjugate into forebrain ventricles to effectively reduce the number of CD163-positive perivascular macrophages with no reduction of food intake after subsequent IL-1beta administration.

See Also:

• Chaskiel L et al. Interleukin-1 reduces food intake and body weight in rat by acting in the arcuate hypothalamus. Brain Behav Immun 81:560-573, 2019.

Hankerd K (2021) Female-specific mechanisms of nociplastic pain in murine model. The University of Texas Medical Branch at Galveston, Dept Neuroscience Thesis.

Objective: To study nociplastic pain the authors developed a murine model in which postinjury thermal stimulation of injured area triggers the transition to a nociplastic pain state more readily in females.

Summary: Postinjury stimulation of an injured area triggers the transition from transient pain to nociplastic pain, females are more susceptible to this transition, and allyl isothiocyanate -sensitive afferents at the previously injured area maintain the nociplastic pain state in a female gonadal hormone-dependent manner.

Usage: Intrathecal injection of Mac-1-SAP (IT-06) 8.85 mM in mice.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

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Borkowski LF, Keilholz AN, Smith CL, Canda KA, Nichols NL (2021) Nonsteroidal anti-inflammatory drug (ketoprofen) delivery differentially impacts phrenic long-term facilitation in rats with motor neuron death induced by intrapleural CTB-SAP injections. Exp Neurol 347:113892. doi: 10.1016/j.expneurol.2021.113892

Objective: To determine the effect of ketoprofen delivery on enhanced phrenic long-term facilitation (pLTF)

Summary: pLTF was surprisingly attenuated in 7d CTB-SAP rats and enhanced in 28d CTB-SAP rats (both p < 0.05) following ketoprofen delivery.

Usage: Rats received bilateral intrapleural injections of CTB-SAP; 25 μg dissolved in PBS.

Related Products: CTB-SAP (Cat. #IT-14)

Schulze J, Staecker H, Wedekind D, Lenarz T, Warnecke A (2022) Expression pattern of brain-derived neurotrophic factor and its associated receptors: Implications for exogenous neurotrophin application. Hear Res 413:108098. doi: 10.1016/j.heares.2020.108098 PMID: 33143996

Objective: To investigate the mechanisms of brain-derived neurotrophic factor (BDNF) in the inner ear, by analyzing the expression of mature BDNF (mBDNF), its proform proBDNF and their respective receptors the low affinity p75 neurotrophin receptor (p75NTR) and the neurotrophic receptor tyrosine kinase 2 (NTRK2).

Summary: Treatment with proBDNF is not toxic for spiral ganglion neuron (SGN) survival but simultaneously not protective. However, combined treatment of mBDNF and proBDNF maintained and perhaps slightly increased the protective effect of mBDNF.

Usage: Immunohistochemistry (1:250); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Joshi R, Batie MR, Fan Q, Varisco BM (2022) Mouse lung organoid responses to reduced, increased, and cyclic stretch. Am J Physiol Lung Cell Mol Physiol 322(1):L162-L173. doi: 10.1152/ajplung.00310.2020 PMID: 34851724

Objective: To test two mouse lung organoid mechanotransductive models to address deficiencies in cell culture models.

Summary: Cyclic stretch increased TGF-β and integrin-mediated signaling, with upstream analysis indicating roles for histone deacetylases, microRNAs, and long noncoding RNAs.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Thorsdottir DJ (2021) The role of BDNF-mediated neuroplasticity in cardiovascular regulation within the hypothalamus and brainstem. Univ Vermont, Dept Pharmacology 1387Thesis.

Summary: This PhD dissertation to determine the mechanism behind BDNF-mediated cardiovascular regulation.

Usage: Rats received bilateral NTS injections of vehicle or Anti-DBH-SAP, which selectively lesions catecholaminergic neurons. Treatment increased blood pressure in the GFP group but failed to affect blood pressure in the BDNF group.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Persaud SP, Ritchey JK, Kim S, Lim S, Ruminski PG, Cooper ML, Rettig MP, Choi J, DiPersio JF (2021) Antibody-drug conjugates plus Janus kinase inhibitors enable MHC-mismatched allogeneic hematopoietic stem cell transplantation. J Clin Invest 131(24):e145501. doi: 10.1172/JCI145501

Objective: To demonstrate that biotinylated anti-CD45-SAP or anti-cKit-SAP mixed with Streptavidin-Saporin along with Janus kinase 1/2, enables alloengraftment on murine allo-hematopoietic stem cell transplantation (HSCT) models.

Summary: HSCT has therapeutic potential. However, the transplantation requires first depletion and secondly, for allogeneic-HCST, host and immune responses need to be controlled to prevent graft rejection. The allo-HSCT conditioning strategy exemplifies the promise of immunotherapy to improve the safety of HSCT for treating hematologic diseases.

Usage: Antibodies were incubated with Streptavidin-ZAP (1:1 molar ratio) for 15 minutes at 20°C and . The doses of CD45.2 and cKit conjugates were injected retroorbitally (41.8 μg and 33.2 μg, respectively).

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Sanna F, Bratzu J, Angioni L, Pina Sorighe M, Cocco C, Argiolas A, Melis MR (2021) Oxytocin-conjugated saporin injected into the substantia nigra of male rats alters the activity of the nigrostriatal dopaminergic system: A behavioral and neurochemical study. Brain Res 1773:147705. doi: 10.1016/j.brainres.2021.147705 PMID: 34744015

Objective: To investigate the effects of Oxytocin-SAP in the substantia nigra of male rats, and assess its impact on nigral Tyrosine Hydroxylase-immunoreactivity, dopamine content, locomotor activity, rotational turning, and striatal dopamine function.

Summary: Researchers investigated the effects of injecting oxytocin-conjugated Saporin into the substantia nigra of male rats. They found that this treatment led to changes in the activity of the nigrostriatal dopaminergic system, as evidenced by alterations in behavior and neurochemical markers associated with dopamine function.

Usage: Oxytocin-SAP (60 ng/μl and 120 ng/μl) was injected unilaterally into the substantia nigra of male rats, respectively, compared to Blank-SAP.

Related Products: Oxytocin-SAP (Cat. #IT-46), Blank-SAP (Cat. #IT-21)