SSP-SAP [IT-11, KIT-11]

a tool for eliminating cells that express substance P receptor (NK-1); targeted via Substance P, eliminated via saporin

SKU: IT-11 Category: Quantity: Individual 25 ug, Individual 100 ug, Kit w/controls 25 ug, Kit w/controls 100 ug | Conjugate: saporin | Usage: eliminates cells |

SSP-SAP is a conjugation of saporin and SSP, the Sar9, Met(O2)11 analog of Substance P. These two amino acid replacements are at two sites of digestion by tissue proteases (there is a third that is still in the analog, so it does eventually get degraded). Since the targeting agent is, of course, necessary for the cytotoxic activity, SSP-SAP will diffuse farther, and thus hit more target cells. This specific analog of SP-saporin resists peptidase digestion, allowing a greater diffusion from the injection site before its metabolism. SSP-SAP eliminates cells expressing the Substance P (NK-1) receptor. Behaviors associated with pain perception are greatly affected by the injection of SSP-SAP into the spinal cord of rats. It is not suitable for retrograde transport.

SSP-SAP is also being used to replace SP-SAP (Cat. #IT-07). Scientific advisors have given counsel for this replacement because SSP-SAP is a superior lesioning agent in many situations, due to its targeting vehicle, a protease-resistant form of substance P. An excellent example is the paper by Martin and Sloviter, J Comp Neurol 436:127-152 (2001), in which after ineffective intraparenchymal injection of SP-SAP in the hippocampus, SSP-SAP was used with tremendous efficacy. In almost all applications we expect SSP-SAP will be used at a lower dose than SP-SAP.

Limited Label License: This product is the subject of claims in a U.S. patent entitled “Substance P-Saporin (SP-SAP) Conjugates and Methods of Use Thereof” (6,063,758 and 7,741,435), owned by Advanced Targeting Systems, Inc. Purchase of this product conveys to the buyer only the non-transferable right under this patent to use the product in research conducted by the buyer.

SSP-SAP is available individually (Cat. #IT-11) or as a kit (Cat. #KIT-11) which includes SSP-SAP and Blank-SAP (Cat. #IT-21).

keywords: saporin, analog, Substance P, SP, NK-1R, NK-1 receptor, NK1R, SSP, Stable Substance-P, pain, peptidease-resistant, interneurons, hippocampus, neocortex, Spinal cord lamina I neurons, brain, neuroscience

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Targeted hippocampal GABA neuron ablation by stable substance P-saporin causes hippocampal sclerosis and chronic epilepsy in rats.

Chun E, Bumanglag AV, Burke SN, Sloviter RS (2019) Targeted hippocampal GABA neuron ablation by stable substance P-saporin causes hippocampal sclerosis and chronic epilepsy in rats. Epilepsia 60(5):e52-e57. doi: 10.1111/epi.14723

Objective: Hippocampal GABA neurons were targeted for selective elimination to determine whether a focal hippocampal GABAergic defect in an otherwise normal brain can initiate cryptogenic temporal lobe epilepsy with hippocampal sclerosis.

Summary: Hippocampal GABAergic dysfunction is epileptogenic and can produce the defining features of cryptogenic temporal lobe epilepsy.

Usage: Intrahippocampal injections of SSP-SAP (0.4 ng/10 nL) were performed using a 0.5-μL Neuros Syringe lowered into four hippocampal sites along both the transverse and longitudinal hippocampal axes bilaterally.

Related Products: SSP-SAP (Cat. #IT-11)

Read the featured article in Targeting Trends.

PARVing the way to cap translation for seizure control

Gross C (2021) PARVing the way to cap translation for seizure control. Epilepsy Curr 21(5):360-362. doi: 10.1177/15357597211027010

Summary: Loss of GABAergic interneurons leads to spontaneous recurrent seizures that persist over months if the amount and spatial spread of initial inhibitory neuron loss is sufficient.

Usage: Intrahippocampal injections of SSP-SAP (0.4 ng/10 nL) were performed using a 0.5-μL Neuros Syringe lowered into four hippocampal sites along both the transverse and longitudinal hippocampal axes bilaterally.

See: Chun E et al. Targeted hippocampal GABA neuron ablation by Stable Substance P-saporin causes hippocampal sclerosis and chronic epilepsy in rats. Epilepsia 60(5):e52-e57, 2019.

Related Products: SSP-SAP (Cat. #IT-11)

Focal inhibitory interneuron loss and principal cell hyperexcitability in the rat hippocampus after microinjection of a neurotoxic conjugate of saporin and a peptidase-resistant analog of substance P.

Martin JL, Sloviter RS (2001) Focal inhibitory interneuron loss and principal cell hyperexcitability in the rat hippocampus after microinjection of a neurotoxic conjugate of saporin and a peptidase-resistant analog of substance P. J Comp Neurol 436:127-152. doi: 10.1002/cne.1065

Usage: The authors used SSP-SAP (0.4 ng/10 nl; Cat. #IT-11).

Related Products: SSP-SAP (Cat. #IT-11)

Read the featured article in Targeting Trends.

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Blank-SAP (Cat. #IT-21)

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