Intrinsically photosensitive retinal ganglion cells (ipRGCs) contain melanopsin. The ipRGCs, with their amazing long processes, are involved in the perception of light and dark and are circadian rhythm determinants. Affinity-purified Anti-Melanopsin (UF008) has very high specificity to mouse ipRGCs.
Affinity-purified anti-Melanopsin (UF008) was raised against the 15 N-terminal extracellular amino acids of mouse melanopsin. This antibody does not cross-react with melanopsins of other species, it is very specific for mouse.
2-photon confocal microscope image from a single z-series using AB-N39 in flat-mount mouse retina at 1:4000and an AlexaFluor-568 secondary antibody. This image is a single optic plane through the ganglion cell layer that also shows some proximal RGC dendrites.
Image courtesy of Matthew Van Hook, Ph.D.
Applications include immunofluorescence (1 µg/ml) and targeting (targeting agent in Melanopsin-SAP, Cat. #IT-44). This antibody works in both cross-section and flat-mount staining.