Contributed by Francesco Ferrini (1) and Yves De Koninck (2,3) 1) Department of Veterinary Sciences, University of Turin,10095 Grugliasco, Turin, Italy 2) Institut universitaire en santé mentale de Québec, QC, G1J 2G3, Canada 3) Department of Psychiatry and Neuroscience, Université Laval, Québec, QC, G13 7P4, Canada Morphine-induced hyperalgesia and tolerance dramatically limit the use of […]
Isolectin B4 (IB4) is a tetrameric protein isolated from Griffonia simplicifolia seeds. Although one of its first uses was the agglutination of B-type red blood cells,[1] it has also been used extensively in the neurosciences because the alpha-galactosyl residues bound by IB4 are present on a variety of cell populations such as the non-peptidergic afferents
Targeting Tools: Recombinant Isolectin B4 (IB4) Read More »
Contributed by Brian J. Russell The newly invigorated BRAIN initiative (Brain Research through Advancing Innovative Neurotechnologies, also known as the Brain Activity Map Project) has placed a premium on understanding the role of each neuron within the human brain. This effort has been our focus for some time via our Targeted Toxin technology. From the
Targeting Article: Targeted Toxins, Optogenetics, and the BRAIN Initiative Read More »
Contributed by Liu Y, Weick JP, Liu H, Krencik R, Zhang X, Ma L, Zhou GM, Ayala M, Zhang SC. Waisman Center, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin, USA. and Human Anatomy and Histology, Fudan University Shanghai Medical School, Shanghai, China. Basal forebrain neurons including cholinergic neurons and GABA interneurons
Background of General Preparation and Components: Over the last few years, riding on the coattails of the established customer base for the ZAP line of secondary conjugates, Fab-ZAP has become the most popular ATS product group in the catalog. Combining species-specific monovalent secondary antibodies with the cytotoxic ribosome-inactivating protein, Saporin, the Fab-ZAP line of products
Targeting Tools: FabFc-ZAP – A new secondary conjugate Read More »
Contributed by Jose Luis Daniotti, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC, UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. Glycosphingolipids (GSLs) are amphipathic molecules consisting of a ceramide lipid moiety linked to a glycan chain of variable length and structure. Among these are found the
Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfuntion of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer’s disease, Down and
Targeting Tools: Anti-ChAT-SAP Read More »
Contributed by François LaPlante. Dept of Psychiatry, McGill University, Montréal, QC, Canada A post-mortem reduction in the density of cholinergic interneurons in the ventral striatum or nucleus accumbens (N.Acc.) has been reported in schizophrenic brains.[1,2] In this region the cholinergic interneurons interact anatomically and functionally with the dopaminergic nerve terminals notably to dampen the effects
Read the 2012 Newsletters and recent scientific references 2012, issue 1: Motivation’s modulation of attention through the mesolimbic-corticopetal cholinergic circuitry / by M St. Peters, M Sarter / featuring 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18) SFN Poster of the Year Targeting Talk: Anti-NGFr blocking antibody, Staining with FITC-labeled Anti-Saporin Targeting Tools: Antibody to Metabotropic Glutamate
Targeting Trends 2012 Newsletter Read More »
The cover article this quarter demonstrates usage of mu p75-SAP (Cat. #IT-16) to study delirium. To create this toxin, we affinity-purified the rabbit polyclonal to the low affinity neurotrophin receptor, p75, (Cat. #AB-N01AP) with the immunogen bound to a solid support, and then conjugated the affinity-purified antibody to saporin (Cat. #PR-01). As can be seen
Targeting Tools: mu p75-SAP Read More »