Targeting Trends 2018 Review

Below are links to view the 2018 annual review newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please subscribe here.


Targeting Trends Newsletter 17q1

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please subscribe here.


The locus coeruleus: a potential link between cerebrovascular and neuronal pathology in Alzheimer’s disease.

Contributed by S C Kelly, P T Nelson, S E Counts

From the President: Accelerating Your Research

The latest addition to the Ancheta family!

Fab-pHAST: The newest targeting tool

Recent Scientific References and Publications

  • Chen SK, Badea TC, Hattar S. (2011) Photoentrainment and Pupillary Light Reflex Are Mediated By Distinct Populations of Iprgcs. Nature 476(7358):92-95. PMID: 21765429 (Cat. #AB-N38)
  • Bourane S, Duan B, Koch SC, Dalet A, Britz O, Garcia-Campmany L, Kim E, Cheng L, Ghosh A, Ma Q, Goulding M. (2015) Gate Control of Mechanical Itch By a Subpopulation of Spinal Cord Interneurons. Science 350(6260):550-54. PMID: 26516282 (Cat. #IT-40)
  • Sorge RE, Mapplebeck JC, Rosen S, Beggs S, Taves S, Alexander JK, Martin LJ, Austin JS, Sotocinal SG, Chen D, Yang M, Shi XQ, Huang H, Pillon NJ, Bilan PJ, Tu Y, Klip A, Ji RR, Zhang J, Salter MW, Mogil JS. (2015) Different Immune Cells Mediate Mechanical Pain Hypersensitivity in Male and Female Mice. Nat Neurosci 18(8):1081-83. PMID: 26120961 (Cat. #IT-06)
  • Somasundaram P, Wyrick GR, Fernandez DC, Ghahari A, Pinhal CM, Simmonds Richardson M, Rupp AC, Cui L, Wu Z, Brown RL, Badea TC, Hattar S, Robinson PR. (2017) C-Terminal Phosphorylation Regulates the Kinetics of a Subset of Melanopsin-Mediated Behaviors in Mice. Proc Natl Acad Sci U S A 114(10):2741-46. PMID: 28223508 (Cat. #AB-N38)
  • Wang L, Conner JM, Nagahara AH, Tuszynski MH. (2016) Rehabilitation Drives Enhancement of Neuronal Structure in Functionally Relevant Neuronal Subsets. Proc Natl Acad Sci U S A 113(10):2750-55. PMID: 26903653 (Cat. #IT-01)
  • Ngo M, Han A, Lakatos A, Sahoo D, Hachey SJ, Weiskopf K, Beck AH, Weissman IL, Boiko AD. (2016) Antibody Therapy Targeting Cd47 and Cd271 Effectively Suppresses Melanoma Metastasis in Patient-Derived Xenografts. Cell Rep 16(6):1701-16. PMID: 27477289 (Cat. #IT-15)
  • Hay CM, Sult E, Huang Q, Mulgrew K, Fuhrmann SR, McGlinchey KA, Hammond SA, Rothstein R, Rios-Doria J, Poon E, Holoweckyj N, Durham NM, Leow CC, Diedrich G, Damschroder M, Herbst R, Hollingsworth RE, Sachsenmeier KF. (2016) Targeting Cd73 in the Tumor Microenvironment With Medi9447. Oncoimmunology 5(8):e1208875. PMID: 27622077 (Cat. #IT-51)
  • Lecrux C, Sandoe CH, Neupane S, Kropf P, Toussay X, Tong XK, Lacalle-Aurioles M, Shmuel A, Hamel E. (2017) Impact of Altered Cholinergic Tones on the Neurovascular Coupling Response to Whisker Stimulation. J Neurosci 37(6):1518-31. PMID: 28069927 (Cat. #IT-01)
  • Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T. (2016) A Non-Inheritable Maternal Cas9-Based Multiple-Gene Editing System in Mice. Sci Rep 6:20011. PMID: 26817415 (Cat. #IT-10)
  • Silva TM, Takakura AC, Moreira TS. (2016) Acute Hypoxia Activates Hypothalamic Paraventricular Nucleus-Projecting Catecholaminergic Neurons in the C1 Region. Exp Neurol 285(Pt A):1-11. PMID: 27569537 (Cat. #IT-03)
  • Xia W, Liu Y. (2016) Targeted Ablation of Cardiac Sympathetic Neurons: A Promising Approach to Prevent Sudden Cardiac Death. Int J Cardiol 202:425-26. PMID: 26433164 (Cat. #IT-14)
  • Turnbull MT, Coulson EJ. (2017) Cholinergic Basal Forebrain Lesion Decreases Neurotrophin Signaling Without Affecting Tau Hyperphosphorylation in Genetically Susceptible Mice. J Alzheimers Dis 55(3):1141-54. PMID: 27767994 (Cat. #IT-16, IT-35)
  • Yuan X, Yang M, Chen X, Zhang X, Sukhadia S, Musolino N, Bao H, Chen T, Xu C, Wang Q, Santoro S, Ricklin D, Hu J, Lin R, Yang W, Li Z, Qin W, Zhao A. (2016) Characterization of the First Fully Human Anti-Tem1 Scfv in Models of Solid Tumor Imaging and Immunotoxin-Based Therapy. Cancer Immunol Immunother PMID: 27933426 (Cat. #IT-27)
  • Benn A, Robinson ES. (2017) Differential Roles for Cortical Versus Sub-Cortical Noradrenaline and Modulation of Impulsivity in the Rat. Psychopharmacology (Berl) 234(2):255-66. PMID: 27744551 (Cat. #IT-03)
  • Cai Y, Chew C, Muñoz F, Sengelaub DR. (2016) Neuroprotective Effects of Testosterone Metabolites and Dependency on Receptor Action on the Morphology of Somatic Motoneurons Following the Death of Neighboring Motoneurons. Dev Neurobiol PMID: 27569375 (Cat. #IT-14)
  • Maass JC, Gu R, Cai T, Wan YW, Cantellano SC, Asprer JS, Zhang H, Jen HI, Edlund RK, Liu Z, Groves AK. (2016) Transcriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearing. PLoS One 11(12):e0167286. PMID: 27918591 (Cat. #AB-N01AP)
  • Iizuka K, Yokomizo T, Watanabe N, Tanaka Y, Osato M, Takaku T, Komatsu N. (2016) Lack of Phenotypical and Morphological Evidences of Endothelial to Hematopoietic Transition in the Murine Embryonic Head During Hematopoietic Stem Cell Emergence. PLoS One 11(5):e0156427. PMID: 27227884 (Cat. #FL-N01AP)
  • Wehner AB, Milen AM, Albin RL, Pierchala BA. (2016) The P75 Neurotrophin Receptor Augments Survival Signaling in the Striatum of Pre-Symptomatic Q175(wt/hd) Mice. Neuroscience 324:297-306. PMID: 26947127 (Cat. #AB-N01AP)
  • Amano M, Amiya N, Yokoyama T, Onikubo K, Yamamoto N, Takahashi A. (2016) Immunohistochemical Detection of Corticotropin-Releasing Hormone (Crh) in the Brain and Pituitary of the Hagfish, Eptatretus Burgeri. Gen Comp Endocrinol 236:174-80. PMID: 27444128 (Cat. #AB-02)
  • Tanigawa M, Suzuki C, Niwano K, Kanekatsu R, Tanaka H, Horiike K, Hamase K, Nagata Y. (2016) Participation of D-Serine in the Development and Reproduction of the Silkworm Bombyx Mori. J Insect Physiol 87:20-29. PMID: 26828952 (Cat. #AB-T049)
  • Jiao J, Xiong W, Wang L, Yang J, Qiu P, Hirai H, Shao L, Milewicz D, Chen YE, Yang B. (2016) Differentiation Defect in Neural Crest-Derived Smooth Muscle Cells in Patients With Aortopathy Associated With Bicuspid Aortic Valves. EBioMedicine 10:282-90. PMID: 27394642 (Cat. #AB-N07)

Talking about Targeting: Neurotransmitter Antibodies

Kermit’s Kollection

Where to See Us:

  • German Neuroscience Society, Göttingen, GERMANY  | March 22-25 | Booth #63
  • Amer Assoc Cancer Research,  Washington, DC USA | April 1-5 | Booth #2554
  • Experimental Biology, Chicago, IL USA | April 22-26 | Booth #462
  • Amer Assoc Immunologists, Washington, DC USA | May 12-16 | Booth #335

Daily Promotions on Targeting Tools: Check our website daily for specials from each of our Product Managers

Targeting Trends Newsletter 16q4

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


The latest edition of Targeting Trends, the quarterly newsletter from Advanced Targeting Systems, is now available online.  For the first time you will be given access and introduced to the strategic partners that ATS entrusts with key aspects of delivering to you the best in targeted conjugate technology and the latest antibodies.  In our ‘Letter from the President’ you can discover whom to contact if you need to source your own targeted conjugate, or outsource assay development and operations to a GLP certified CRO, or simply handle global shipment logistics for your growing company.

Our cover article, Targeted lesioning reveals role of nucleus incertus in the anxiogenic effect of buspirone, continues the Dawe’s lab’s work with CRF-SAP in the nucleus incertus and offers greater clarity on the role of buspirone in anxiety modulation.  We have also added to the team here at ATS, so check out Targeting Talk to learn about our newest Product Manager and what she’s working on, as well as a sneak preview of new products coming soon.

As always, our feline ATS mascot has been up to his usual mischief with the photos to prove it.

Thank you for your continued support and please contact us if there is anything we can do to improve your ATS experience.

With kind regards,

Brian Russell, Editor

Targeted lesioning reveals role of nucleus incertus in the anxiogenic effect of buspirone

By Jigna Rajesh Kumar, [a, b, c, d] Ramamoorthy Rajkumar, [a, b, c] Liying Corinne Lee, [a, b, c] Gavin S. Dawe [a, b, c, d] [a] Dept Pharmacology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 117600, Singapore [b] Neurobiology and Ageing Programme, Life Sciences Institute, National University of Singapore, 117456, Singapore [c] Singapore Institute for Neurotechnology (SINAPSE), 117456, Singapore [d] NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 117456, Singapore

From the President: Strategic Partners

Recent Scientific References and Publications

  • Lim JH, Stafford BK, Nguyen PL, Lien BV, Wang C, Zukor K, He Z, Huberman AD. (2016) Neural activity promotes long-distance, target-specific regeneration of adult retinal axons. Nat Neurosci 19(8):1073-1084. PMID: 27399843 (Cat. #AB-N38)
  • Sedlik C, Heitzmann A, Viel S, Ait Sarkouh R, Batisse C, Schmidt F, De La Rochere P, Amzallag N, Osinaga E, Oppezzo P, Pritsch O, Sastre-Garau X, Hubert P, Amigorena S, Piaggio E. (2016) Effective antitumor therapy based on a novel antibody-drug conjugate targeting the Tn carbohydrate antigen. Oncoimmunology 5(7):e1171434. PMID: 27622021 (Cat. #PR-01, Custom)
  • Lee SJ, Diener K, Kaufman S, Krieger JP, Pettersen KG, Jejelava N, Arnold M, Watts AG, Langhans W. (2016) Limiting glucocorticoid secretion increases the anorexigenic property of Exendin-4. Mol Metab 5(7):552-565. PMID: 27408779 (Cat. #IT-03, Cat. #PR-01)
  • Webster CI, Caram-Salas N, Haqqani AS, Thom G, Brown L, Rennie K, Yogi A, Costain W, Brunette E, Stanimirovic DB. (2016) Brain penetration, target engagement, and disposition of the blood-brain barrier-crossing bispecific antibody antagonist of metabotropic glutamate receptor type 1. FASEB J 30(5):1927-1940. PMID: 26839377 (Cat. #AB-213)
  • Akagi S, Kono N, Ariyama H, Shindou H, Shimizu T, Arai H. (2016) Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids. FASEB J 30(5):2027-2039. PMID: 26887439 (Cat. #AB-259)
  • Coradazzi M, Gulino R, Fieramosca F, Falzacappa LV, Riggi M, Leanza G. (2016) Selective noradrenaline depletion impairs working memory and hippocampal neurogenesis. Neurobiol Aging 48:93-102. PMID: 27644078 (Cat. #IT-03)
  • Kumar JR, Rajkumar R, Lee LC, Dawe GS. (2016) Nucleus incertus contributes to an anxiogenic effect of buspirone in rats: Involvement of 5-HT1A receptors. Neuropharmacology 110(Pt A):1-14. PMID: 27436722 (Cat. #IT-13, Cat. #IT-21)
  • Mao CA, Agca C, Mocko-Strand JA, Wang J, Ullrich-Luter E, Pan P, Wang SW, Arnone MI, Frishman LJ, Klein WH. (2016) Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. Proc Biol Sci 283(1826):20152978. PMID: 26962139 (Cat. #AB-N39)
  • Kaushal R, Taylor BK, Jamal AB, Zhang L, Ma F, Donahue R, Westlund KN. (2016) GABA-A receptor activity in the noradrenergic locus coeruleus drives trigeminal neuropathic pain in the rat; contribution of NAalpha1 receptors in the medial prefrontal cortex. Neuroscience 334:148-159. PMID: 27520081 (Cat. #IT-03, Cat. #IT-18)
  • Nam H, Kerman IA. (2016) A2 noradrenergic neurons regulate forced swim test immobility. Physiol Behav 165:339-349. PMID: 27553574 (Cat. #IT-03)
  • Gilabert-Oriol R, Thakur M, Haussmann K, Niesler N, Bhargava C, Gorick C, Fuchs H, Weng A. (2016) Saponins from Saponaria officinalis L. Augment the Efficacy of a Rituximab-Immunotoxin. Planta Med PMID: 27392242 (Cat. #IT-37, Cat. #IT-24)

Targeting Toolbox: New Product Manager Alena Bishop

Kermit’s Kollection

Daily Promotions on Targeting Tools: Check our website daily for specials from each of our Product Managers

Targeting Trends Newsletter 16q3

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


The latest edition of Targeting Trends is now available online. The cover article, “Targeted depletion of hematopoietic stem cells promises safer transplantation” demonstrates the powerful capabilities of Streptavidin-ZAP for in vivo applications. We appreciate Dr. Palchaudhuri sharing a summary of his laboratory’s results published in Nature Biotechnology. The Targeting Talk section of the newsletter provides more information about the secondary conjugate system.   The focus of Targeting Tools in this issue is on the Orexin family of products, targeting either OX1R or OX2R expressing cells. The Letter from the President explains the difference between ZAP products and SAP products. A summary of the recent publications using ATS products, a special picture of Kermit, and a summary of this month’s promotions are all included in this latest issue.  Enjoy and pass it on.

Targeted depletion of hematopoietic stem cells promises safer transplantation

By Rahul Palchaudhuri, Ph.D., Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA

From the President: What’s ZAP

Targeting Toolbox:

Recent Scientific References and Publications

  • Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes DB, Tate TA, Czechowicz A, Kfoury Y, Ruchika F, Rossi DJ, Verdine GL, Mansour MK, Scadden DT. (2016) Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34(7):738-745. PMID: 27272386 (Cat. #IT-27)
  • Arroyo DA, Kirkby LA, Feller MB. (2016) Retinal Waves Modulate an Intraretinal Circuit of Intrinsically Photosensitive Retinal Ganglion Cells. J Neurosci 36(26):6892-6905. PMID: 27358448 (Cat. #AB-N38)
  • Araldi D, Ferrari LF, Levine JD. (2016) Gi-Protein Coupled 5-HT1B/D Receptor Agonist Sumatriptan Induces Type I Hyperalgesic Priming. Pain PMID: 27075428 (Cat. #IT-10, IT-11)
  • Yao Y, Echeverry S, Shi XQ, Yang M, Yang QZ, Wang GY, Chambon J, Wu YC, Fu KY, De Koninck Y, Zhang J. (2016) Dynamics of spinal microglia repopulation following an acute depletion. Sci Rep 6:22839. PMID: 26961247 (Cat. #IT-06)
  • Cetas JS, McFarlane R, Kronfeld K, Smitasin P, Liu JJ, Raskin JS. (2015) Brainstem opioidergic system is involved in early response to experimental SAH. Transl Stroke Res 6(2):140-147. PMID: 25417789 (Cat. #IT-12, IT-21)
  • Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. (2016) Ablation of KNDy neurons results in hypogonadotropic hypogonadism and amplifies the steroid-induced LH surge in female rats. Endocrinology 157(5):2015-2027. PMID: 26937713 (Cat. #IT-63, IT-21)
  • Angioni L, Cocco C, Ferri GL, Argiolas A, Melis MR, Sanna F. (2016) Involvement of nigral oxytocin in locomotor activity: A behavioral, immunohistochemical and lesion study in male rats. Horm Behav 83:23-38. PMID: 27189764 (Cat. #IT-46, IT-21)
  • Matsuura T, Kawasaki M, Hashimoto H, Yoshimura M, Motojima Y, Saito R, Ueno H, Maruyama T, Ishikura T, Sabanai K, Mori T, Ohnishi H, Onaka T, Sakai A, Ueta Y. (2016) Possible Involvement of the Rat Hypothalamo-Neurohypophysial/-Spinal Oxytocinergic Pathways in Acute Nociceptive Responses. J Neuroendocrinol 28(6) PMID: 27144381 (Cat. #IT-46, IT-21)
  • Mohammed M, Kulasekara K, Ootsuka Y, Blessing WW. (2016) Locus coeruleus noradrenergic innervation of the amygdala facilitates alerting-induced constriction of the rat tail artery. Am J Physiol Regul Integr Comp Physiol 310(11):R1109-19. PMID: 27101292 (Cat. #IT-03, IT-20)
  • Gritsch S, Bali KK, Kuner R, Vardeh D. (2016) Functional characterization of a mouse model for central post-stroke pain. Molecular Pain 12:1744806916629049. PMID: (Cat. #IT-11, PR-01)
  • Fei Y, Wang X, Chen S, Zhou Q, Zhang C, Li Y, Sun L, Zhang L. (2016) Role of the RVM in Descending Pain Regulation Originating from the Cerebrospinal Fluid-Contacting Nucleus. Neurochem Res 41(7):1651-1661. PMID: 26961890 (Cat. #IT-14)
  • Matsuura T, Kawasaki M, Hashimoto H, Yoshimura M, Motojima Y, Saito R, Ueno H, Maruyama T, Sabanai K, Mori T, Ohnishi H, Sakai A, Ueta Y. (2016) Effects of central administration of oxytocin-saporin cytotoxin on chronic inflammation and feeding/drinking behaviors in adjuvant arthritic rats. Neurosci Lett 621:104-110. PMID: 27060190 (Cat. #IT-46, IT-21)

Talking About Targeting: Streptavidin-ZAP Applications

Kermit’s Kollection

Daily Promotions on Targeting Tools: Check our website daily for specials from each of our Product Managers

Targeting Trends Newsletter 16q2

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


The latest edition of Targeting Trends, the quarterly newsletter from Advanced Targeting Systems, is now available online.  I hope you will enjoy our new look as we continue to harmonize our digital platform, from the redesigned website to our e-commerce promotions and this periodical.  Our cover article, Cerebral cholinergic lesion reduces operant responses to unpleasant thermal stimuli, further emphasizes the profound effects and uses of 192-IgG-SAP as a lesioning tool and is contributed by two experts in the use of Saporin conjugates, Drs. Ron Wiley and Charles Vierck.

Along with the new release of vesicular GABA transporter (vGAT)-related products and our new stable transfected cell lines, our Letter from the President addresses the exciting work of human sighs and the researcher that’s ‘gone viral.’  For the next installment of a long and ongoing discussion about the safety of Saporin in the lab and clinic, check out Targeting Talk. Lastly, our feline ATS mascot has initiated a new contest with hundreds of dollars of product credit at stake!

Cerebral cholinergic lesion reduces operant responses to unpleasant thermal stimuli

by Ronald G. Wiley, M.D., Ph.D., Departments of Neurology and Pharmacology, Vanderbilt University, Nashville, TN  and C. J. Vierck, Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL, USA

From the President: A Sigh is (Not) Just a Sigh . . .

Targeting Toolbox:

Recent Scientific References and Publications

  • Li P, Janczewski WA, Yackle K, Kam K, Pagliardini S, Krasnow MA, Feldman JL. (2016) The peptidergic control circuit for sighing. Nature 530(7590):293-297. PMID: 26855425 (Cat. #IT-40, Cat. #IT-21)
  • Vierck CJ, Yezierski RP, Wiley RG. (2016) Pain sensitivity following loss of cholinergic basal forebrain (CBF) neurons in the rat. Neuroscience 319:23-34. PMID: 26812034 (Cat. #IT-01)
  • Fu R, Chen X, Zuo W, Li J, Kang S, Zhou LH, Siegel A, Bekker A, Ye JH. (2016) Ablation of mu opioid receptor-expressing GABA neurons in rostromedial tegmental nucleus increases ethanol consumption and regulates ethanol-related behaviors. Neuropharmacology 107:58-67. PMID: 26921770 (Cat. #IT-12, Cat. #IT-21)
  • Mao CA, Agca C, Mocko-Strand JA, Wang J, Ullrich-Lüter E, Pan P, Wang SW, Arnone MI, Frishman LJ, Klein WH. (2016) Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. Proc R Soc B 283(1826):20152978.  (Cat. #AB-N39)
  • Yaksh TL, Fisher C, Hockman T, Wiese A. (2016) Current and Future Issues in the development of spinal agents for the management of pain. Curr Neuropharmacol PMID: 26861470 (Cat. #IT-11)
  • Hulsey DR, Hays SA, Khodaparast N, Ruiz A, Das P, Rennaker RLn, Kilgard MP. (2016) Reorganization of Motor Cortex by Vagus Nerve Stimulation Requires Cholinergic Innervation. Brain Stimul 9(2):174-181. PMID: 26822960 (Cat. #IT-01, Cat. #IT-18)
  • Lee JE, Jeong da U, Lee J, Chang WS, Chang JW. (2016) The effect of nucleus basalis magnocellularis deep brain stimulation on memory function in a rat model of dementia. BMC Neurol 16(1):6. PMID: 26757896 (Cat. #IT-01)
  • Gulino R. (2016) Neuroplasticity and Repair in Rodent Neurotoxic Models of Spinal Motoneuron Disease. Neural Plast 2016:2769735. PMID: 26862439 (Cat. #IT-14)
  • Nohara S, Kato K, Fujiwara D, Sakuragi N, Yanagihara K, Iwanuma Y, Kajiyama Y. (2016) Aminopeptidase N (APN/CD13) as a target molecule for scirrhous gastric cancer. Clin Res Hepatol Gastroenterol PMID: 26774363 (Cat. #IT-04)
  • Petrosini L, De Bartolo P, Cutuli D, Gelfo F. (2016) Perinatal 192 IgG-Saporin as Neuroteratogen. Curr Top Behav Neurosci 29:111-123. PMID: 26695170 (Cat. #IT-01)
  • Archer T, Kostrzewa RM. (2016) Neuroteratology and Animal Modeling of Brain Disorders. Curr Top Behav Neurosci 29:1-40. PMID: 26857462 (Review)
  • Schwartz MD, Nguyen AT, Warrier DR, Palmerston JB, Thomas AM, Morairty SR, Neylan TC, Kilduff TS. (2016) Locus Coeruleus and Tuberomammillary Nuclei Ablations Attenuate Hypocretin/Orexin Antagonist-Mediated REM Sleep. eNeuro 3(2) PMID: 27022631 (Cat. #IT-03, IT-20)

Creative Captions: Send us your caption for Kermit’s hijinks.
The winning caption will be posted in the next newsletter and the author will receive a $350 product credit, runner-up will receive $100 product credit.

Talking About Targeting: Saporin Safety – Nothing to Fear

Daily Promotions on Targeting Tools: Check our website daily for specials from each of our Product Managers

Targeting Trends Newsletter 16q1

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


Dermorphin-SAP is highlighted this quarter in the cover article “Striatal patch compartment lesions reduce cocaine-induced repetitive behaviors” by by Kristen Ashley Horner and collaborators R. Murray and M.C. Logan. Read about the “New Beginnings” at ATS in the Message from the President. And learn more about great “Targeting Tools” including Custom Conjugates, Beta Products and the brand new website.

“Targeting Talk” answers questions about safety of toxins in conjugates and the latest articles using ATS products are highlighted in Recent Scientific References. Make sure to check the website daily for promotions and follow us on LinkedIn and Twitter!

Striatal patch compartment lesions reduce cocaine-induced repetitive behaviors
(continued on page 6)

by Kristen Ashley Horner and collaborators R. Murray and M.C. Logan Mercer University School of Medicine, Savannah, GA 31404

Message from the President: New Beginnings

Upcoming Events

  • American Academy of Pain Medicine / Feb 18-21, 2016 / Palm Springs, CA
  • Experimental Biology / April 2-6, 2016 / San Diego, CA

Targeting Teaser Winners

Recent Scientific References (read summaries)

  • Navratilova E, Xie JY, Meske D, Qu C, Morimura K, Okun A, Arakawa N, Ossipov M, Fields HL, Porreca F. (2015) Endogenous opioid activity in the anterior cingulate cortex is required for relief of pain. J Neurosci 35(18):7264-7271. (Cat. #IT-12, PR-01)
  • Gibon J, Buckley SM, Unsain N, Kaartinen V, Seguela P, Barker PA. (2015) proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons. J Neurosci 35(26):9741-9753. (Cat. #AB-N01)
  • Araldi D, Ferrari LF, Levine JD. (2015) Repeated Mu-Opioid Exposure Induces a Novel Form of the Hyperalgesic Priming Model for Transition to Chronic Pain. J Neurosci 35(36):12502-12517. (Cat. #IT-10)
  • La JH, Feng B, Kaji K, Schwartz ES, Gebhart GF. (2016) Roles of isolectin B4-binding afferents in colorectal mechanical nociception. Pain 157(2):348-354. (Cat. #IT-10, PR-01)
  • Riazifar H, Sun G, Wang X, Rupp A, Vemaraju S, Ross-Cisneros FN, Lang RA, Sadun AA, Hattar S, Guan MX, Huang T. (2015) Phenotypic and functional characterization of Bst+/- mouse retina. Dis Model Mech 8(8):969-976. (Cat. #AB-N38)
  • Arora V, Morado-Urbina CE, Aschenbrenner CA, Hayashida KI, Wang F, Martin TJ, Eisenach JC, Peters CM. (2016) Disruption of Spinal Noradrenergic Activation Delays Recovery of Acute Incision-Induced Hypersensitivity and Increases Spinal Glial Activation in the Rat. J Pain 17(2):190-202. (Cat. #IT-03, IT-18)
  • Aicher SA, Hermes SM, Hegarty DM. (2015) Denervation of the Lacrimal Gland Leads to Corneal Hypoalgesia in a Novel Rat Model of Aqueous Dry Eye Disease. Invest Ophthalmol Vis Sci 56(11):6981-6989. (Cat. #IT-01)
  • Koppen JR, Stuebing SL, Sieg ML, Blackwell AA, Blankenship PA, Cheatwood JL, Wallace DG. (2016) Cholinergic deafferentation of the hippocampus causes non-temporally graded retrograde amnesia in an odor discrimination task. Behav Brain Res 299:97-104. (Cat. #IT-01)
  • Devoto P, Flore G, Saba P, Frau R, Gessa GL. (2015) Selective inhibition of dopamine-beta-hydroxylase enhances dopamine release from noradrenergic terminals in the medial prefrontal cortex. Brain Behav 5(10):e00393. (Cat. #IT-03, IT-18, PR-01)
  • Chen Y, Pan C, Xuan A, Xu L, Bao G, Liu F, Fang J, Long D. (2015) Treatment Efficacy of NGF Nanoparticles Combining Neural Stem Cell Transplantation on Alzheimer’s Disease Model Rats. Med Sci Monit 21:3608-3615. (Cat. #IT-01)
  • Jeong da U, Oh JH, Lee JE, Lee J, Cho ZH, Chang JW, Chang WS. (2016) Basal Forebrain Cholinergic Deficits Reduce Glucose Metabolism and Function of Cholinergic and GABAergic Systems in the Cingulate Cortex. Yonsei Med J 57(1):165-172. (Cat. #IT-01)
  • Dickey DD, Thomas GS, Dassie JP, Giangrande PH. (2016) Method for Confirming Cytoplasmic Delivery of RNA Aptamers. Methods Mol Biol 1364:209-217. (Cat. #IT-27, IT-38)

Targeting Talk: Product Questions

  • Q: Our QA group wants to know about the safety of the toxin in your conjugates? What precautions should we take in handling saporin products?[see answer]

Contact your Local Distributor to place an order today

Striatal patch compartment lesions reduce cocaine-induced repetitive behaviors
(continued from page 1)

Custom Conjugates: Made to Order

Beta Products

Brand New Website

Kermit’s Kollection

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Targeting Trends Newsletter 15q4

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


Anti-ChAT-SAP is highlighted this quarter in the cover article “A specific immunotoxin elucidates a causal role of striatal cholinergic system in behavioral flexibility” and in “Targeting Tools.” The ATS Product Managers are also pleased to introduce two new products: MonoBiotin-ZAP and PACAP-SAP.

“Targeting Talk” answers questions about retrograde transport and where to find the citation Suicide Transport and Immunolesioning. The latest articles using ATS products are highlighted in Recent Scientific References and solve the latest “Targeting Teaser” to win a $100 product credit!

A specific immunotoxin elucidates a causal role of striatal cholinergic system in behavioral flexibility
(continued on page 6)

by Sho Aoki and Jeffery R. Wickens Neurobiology Research Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Kunigami, Okinawa 904-0495, Japan

New Product: MonoBiotin-ZAP (Cat. #BT-ZAP)

Upcoming Events

  • Society for Neuroscience / October 17-21, 2015 / Chicago, IL / Booth #662
  • Experimental Biology / April 2-6, 2016 / San Diego, CA

Targeting Teaser Winners

Recent Scientific References (read summaries)

  • Harasawa I, Johansen JP, Fields HL, Porreca F, Meng ID. (2016) Alterations in the rostral ventromedial medulla after the selective ablation of mu-opioid receptor expressing neurons. Pain 157(1):166-173. (Cat. #IT-12, Cat. #PR-01)
  • Cao Q, Lu J, Li Q, Wang C, Wang XM, Lee VW, Wang C, Nguyen H, Zheng G, Zhao Y, Alexander SI, Wang Y, Harris DC. (2016) CD103+ Dendritic Cells Elicit CD8+ T Cell Responses to Accelerate Kidney Injury in Adriamycin Nephropathy. J Am Soc Nephrol 27(5):1344-1360. (Cat. #IT-50, Cat. #IT-17)
  • Damelin M, Bankovich A, Park A, Aguilar J, Anderson W, Santaguida M, Aujay M, Fong S, Khandke K, Pulito V, Ernstoff E, Escarpe P, Bernstein J, Pysz M, Zhong W, Upeslacis E, Lucas J, Lucas J, Nichols T, Loving K, Foord O, Hampl J, Stull R, Barletta F, Falahatpisheh H, Sapra P, Gerber HP, Dylla SJ. (2015) Anti-EFNA4 Calicheamicin Conjugates Effectively Target Triple-Negative Breast and Ovarian Tumor-Initiating Cells to Result in Sustained Tumor Regressions. Clin Cancer Res 21(18):4165-4173. (Cat. #IT-27)
  • Helena CV, Toporikova N, Kalil B, Stathopoulos AM, Pogrebna VV, Carolino RO, Anselmo-Franci JA, Bertram R. (2015) KNDy neurons modulate the magnitude of the steroid-induced luteinizing hormone surges in ovariectomized rats. Endocrinology 156(11):4200-4213. (Cat. #IT-63)
  • Pires ES, D’Souza RS, Needham MA, Herr AK, Jazaeri AA, Li H, Stoler MH, Anderson-Knapp KL, Thomas T, Mandal A, Gougeon A, Flickinger CJ, Bruns DE, Pollok BA, Herr JC. (2015) Membrane associated cancer-oocyte neoantigen SAS1B/ovastacin is a candidate immunotherapeutic target for uterine tumors. Oncotarget 6(30):30194-30211. (Cat. #IT-57, Cat. #IT-35)
  • Buhr ED, Yue WW, Ren X, Jiang Z, Liao HR, Mei X, Vemaraju S, Nguyen MT, Reed RR, Lang RA, Yau KW, Van Gelder RN. (2015) Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea.Proc Natl Acad Sci U S A 112(42):13093-13098 (Cat. #AB-N38)
  • Garcia-Castillo MD, Tran T, Bobard A, Renard HF, Rathjen SJ, Dransart E, Stechmann B, Lamaze C, Lord M, Cintrat JC, Enninga J, Tartour E, Johannes L. (2015) Retrograde transport is not required for cytosolic translocation of the B-subunit of Shiga toxin. J Cell Sci 128(13):2373-2387. (Custom)
  • King TL, Ruyle BC, Kline DD, Heesch CM, Hasser EM. (2015) Catecholaminergic neurons projecting to the paraventricular nucleus of the hypothalamus are essential for cardiorespiratory adjustments to hypoxia. Am J Physiol Regul Integr Comp Physiol 309(7):R721-31. (Cat. #IT-03, Cat. #IT-18)
  • Freiria-Oliveira AH, Blanch GT, Pedrino GR, Cravo SL, Murphy D, Menani JV, Colombari DS. (2015) Catecholaminergic neurons in the comissural region of the nucleus of the solitary tract modulate hyperosmolality-induced responses. Am J Physiol Regul Integr Comp Physiol 309(9):R1082-91. (Cat. #IT-03, Cat. #IT-18)
  • Saeed AW, Pawlowski SA, Ribeiro-da-Silva A. (2015) Limited changes in spinal lamina I dorsal horn neurons following the cytotoxic ablation of non-peptidergic C-fibers. Mol Pain 11(1):54. (Cat. #IT-10)
  • Oyamaguchi A, Abe T, Sugiyo S, Niwa H, Takemura M. (2016) Selective elimination of isolectin B4-binding trigeminal neurons enhanced formalin-induced nocifensive behavior in the upper lip of rats and c-Fos expression in the trigeminal subnucleus caudalis. Neurosci Res 103:40-47. (Cat. #IT-10, Cat. #IT-21)
  • McHugh SB, Francis A, McAuley JD, Stewart AL, Baxter MG, Bannerman DM. (2015) Hippocampal acetylcholine depletion has no effect on anxiety, spatial novelty preference, or differential reward for low rates of responding (DRL) performance in rats. Behav Neurosci 129(4):491-501. (Cat. #IT-01)
  • Bourassa EA, Stedenfeld KA, Sved AF, Speth RC. (2015) Selective C1 Lesioning Slightly Decreases Angiotensin II Type I Receptor Expression in the Rat Rostral Ventrolateral Medulla (RVLM). Neurochem Res 40(10):2113-2120. (Cat. #IT-03, Cat. #IT-18)
  • Kras JV, Weisshaar CL, Pall PS, Winkelstein BA. (2015) Pain from intra-articular NGF or joint injury in the rat requires contributions from peptidergic joint afferents. Neurosci Lett 604:193-198. (Cat. #IT-10, Cat. #IT-11, Cat. #PR-01)

Targeting Talk: Product Questions

  • Hello, I have used your Anti-DBH-SAP (Cat. #IT-03) conjugate, and I’m having a hard time finding this citation: R.G. Wiley, D.A. Lappi. Suicide Transport and Immunolesioning. Molecular Biology Intelligence Unit, R.G. Landes Co, Austin, TX (1994). Do you know where I could find a copy? [see answer]
  • I’m trying to find out if enough Anti-DBH-SAP will be retrogradely transported and taken up by non targeted sympathetic neurons by bulk fluid-phase endocytosis. Does saporin become degraded after it kills the neuron or does it enter the extracellular matrix? [see answer]

Recent Scientific References (read summaries)

Anti-ChAT-SAP elucidates a causal role in behavioral flexibility
(continued from page 1)

Anti-ChAT-SAP

NEW Beta Product: PACAP-SAP

Kermit’s Kollection

Targeting Technology Tutorial

Targeting Teaser (Jumble) – Win a $100 product credit!

Targeting Trends Newsletter 15q3

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


SP-SAP Human Clinical Trial for Cancer Pain is the cover article this quarter discussing the positive feedback from current patients along with the efficacy and drug delivery from an anesthesiologists point of view (Dr. Carl Noe). Our team of Product Managers highlight their products and the Beta Testing Program continues to be a great opportunity at a very low price.

“Targeting Talk” answers questions about incubating primary antibodies and which controls to use. The latest articles using ATS products are highlighted in Recent Scientific References and solve the latest “Targeting Teaser” to win a $100 product credit!

SP-SAP Human Clinical Trial for Cancer Pain – An Anesthesiologist’s Point of View
(continued on page 6)

by Carl Noe, M.D., Anesthesiology & Pain Management, UT Southwestern Medical Center, Dallas, TX; and Eugene McDermott Center for Pain Management, Dallas, Texas

Product Managers Highlight Their Products

Meet LUCAS CHANCE – No, he’s not a Product Manager… yet! Welcome to Lucas Ancheta (son to Leonardo and Kate Ancheta), born April 14, 2015, weighing in at 5 lb, 2 oz.

Targeting Teaser Winners

Recent Scientific References (read summaries)

  • Xu M, Kobets A, Du JC, Lennington J, Li L, Banasr M, Duman RS, Vaccarino FM, DiLeone RJ, Pittenger C. (2015) Targeted ablation of cholinergic interneurons in the dorsolateral striatum produces behavioral manifestations of Tourette syndrome. Proc Natl Acad Sci U S A 112(3):893-898. (Cat. #IT-42, Cat. #IT-35)
  • Aoki S, Liu AW, Zucca A, Zucca S, Wickens JR. (2015) Role of striatal cholinergic interneurons in set-shifting in the rat. J Neurosci 35(25):9424-9431. (Cat. #IT-42)
  • Akiyama T, Nguyen T, Curtis E, Nishida K, Devireddy J, Delahanty J, Carstens MI, Carstens E. (2015) A central role for spinal dorsal horn neurons that express neurokinin-1 receptors in chronic itch. Pain 156(7):1240-1246. (Cat. #IT-40, Cat. #IT-11, Cat. #IT-21)
  • Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. (2015) Neurokinin 3 Receptor-Expressing Neurons in the Median Preoptic Nucleus Modulate Heat-Dissipation Effectors in the Female Rat. Endocrinology 156(7):2552-2562. (Cat. #IT-63, Cat. #IT-21)
  • Li AJ, Wang Q, Elsarelli MM, Brown RL, Ritter S. (2015) Hindbrain catecholamine neurons activate orexin neurons during systemic glucoprivation in male rats. Endocrinology 156(8):2807-2820. (Cat. #IT-03, Cat. #PR-01)
  • Li AJ, Wang Q, Davis H, Wang R, Ritter S. (2015) Orexin-A Enhances Feeding in Male Rats by Activating Hindbrain Catecholamine Neurons. Am J Physiol Regul Integr Comp Physiol Epub:ajpregu.00065.2015. (Cat. #IT-03, Cat. #PR-01)
  • Burke PG, Kanbar R, Viar KE, Stornetta RL, Guyenet PG. (2015) Selective optogenetic stimulation of the retrotrapezoid nucleus in sleeping rats activates breathing without changing blood pressure or causing arousal or sighs. J Appl Physiol (1985) 118(12):1491-1501. (Cat. #IT-03)
  • Xing D, Wu Y, Li G, Song S, Liu Y, Liu H, Wang X, Fei Y, Zhang C, Li Y, Zhang L. (2015) Role of cerebrospinal fluid-contacting nucleus in sodium sensing and sodium appetite. Physiol Behav 147:291-299. (Cat. #IT-14)
  • Wang B, Miao Y, Zhao Z, Zhong Y. (2015) Inflammatory Macrophages Promotes Development of Diabetic Encephalopathy. Cell Physiol Biochem 36(3):1142-1150. (Cat. #IT-06)
  • Murray RC, Logan MC, Horner KA. (2015) Striatal patch compartment lesions reduce stereotypy following repeated cocaine administration. Brain Res Epub(Cat. #IT-12, Cat. #PR-01)
  • Wu YH, Song SY, Liu H, Xing D, Wang X, Fei Y, Li GL, Zhang C, Li Y, Zhang LC. (2015) Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress. Neuropeptides 51:43-54. (Cat. #IT-14, Cat. #PR-01)
  • Pergolizzi JV, Gharibo C, Ho KY. (2014) Treatment Considerations for Cancer Pain: A Global Perspective. Pain Pract Epub (Cat. #IT-07)
  • Gulino R, Parenti R, Gulisano M. (2015) Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease. Biomed Res Int 2015:654637. (Cat. #IT-14)
  • Dashniani M, Kruashvili L, Rusadze Kh, Mataradze S, Beselia G. (2015) Effects of immunotoxic and electrolytic lesions of medial septal area on spatial short-term memory in rats. Georgian Med News (239):98-103. (Cat. #IT-01, Cat. #IT-32, Cat. #PR-01)
  • Burjanadze M, Mataradze S, Rusadze K, Chkhikvishvili N, Dashniani M. (2015) Selective lesion of gaba-ergic neurons in the medial septum by gat1-saporin impairs spatial learning in a water-maze. Georgian Med News (240):59-64. (Cat. #IT-32, Cat. #PR-01)
  • Dashniani MG, Burjanadze MA, Naneishvili TL, Chkhikvishvili NC, Beselia GV, Kruashvili LB, Pochkhidze NO, Chighladze MR. (2015) Exploratory behavior and recognition memory in medial septal electrolytic, neuro- and immunotoxic lesioned rats. Physiol Res Epub (Cat. #IT-01, Cat. #PR-01)

Targeting Talk: Product Questions

  • For in vitro cytotoxicity assays, could you tell me: 1) whether you incubate primary with your Saporin secondary for a specific amount of time prior to cell addition, and 2) do you use a single concentration of secondary per well or a primary:secondary ratio — like 1:2 or 1:4? [see answer]
  • Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? [see answer]
  • Which control is best to use with Octreotide-SAP? [see answer]

SP-SAP Human Clinical Trial for Cancer Pain
(continued from page 1)

Upcoming Events

  • Pain & Migraine Therapeutics Summit / September 23-24, 2015 / Washington, DC
  • Society for Neuroscience / October 17-21, 2015 / Chicago, IL / Booth #662

Beta Products. Great Opportunity for Discovery at a Very Low Price

Kermit’s Kollection

Targeting Technology Tutorial

Targeting Teaser (Jumble) – Win a $100 product credit!

Targeting Trends Newsletter 15q2

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


Drug-free selection of stable transfectants using rIB4-SAP is the cover article this quarter featuring rIB4-SAP and contributed by Drs. Sato and Watanabe. Make sure you check out our Beta Testing Program. It is a great price and and great opportunity for new products. ATS is also pleased to offer Custom Biotinylation Service — when the “one-step-kit” doesn’t quite meet your needs.

“Targeting Talk” answers questions about dissolving targeted toxins, Anti-DBH-SAP, and flow cytometry services. The latest articles using ATS products are highlighted in Recent Scientific References and the “Targeting Teaser” from last quarter has been corrected. Solve to win a jigsaw puzzle featuring your favorite knockout mouse.

Drug-free selection of stable transfectants using targeted toxin technology and a vector expressing cell-surface carbohydrate-digesting enzyme
(continued on page 6)

by Masahiro Sato (Ph.D.) and Satoshi Watanabe (Ph.D.)

Related products: rIB4-SAP (Cat. #IT-10)

Beta Testing Program: Great Price/Great Opportunity

Targeting Teaser Correction – Last quarter’s puzzle was posted incorrectly. We are sorry for any inconvenience. It has been corrected and can be solved online. Win a jigsaw puzzle!

Recent Scientific References (read summaries)

  • Kim T, Thankachan S, McKenna JT, McNally JM, Yang C, Choi JH, Chen L, Kocsis B, Deisseroth K, Strecker RE, Basheer R, Brown RE, McCarley RW. (2015) Cortically projecting basal forebrain parvalbumin neurons regulate cortical gamma band oscillations. Proc Natl Acad Sci U S A 112(11):3535-3540. (Cat. #IT-16)
  • Devesa I, Ferrandiz-Huertas C, Mathivanan S, Wolf C, Lujan R, Changeux JP, Ferrer-Montiel A. (2014) alphaCGRP is essential for algesic exocytotic mobilization of TRPV1 channels in peptidergic nociceptors. Proc Natl Acad Sci U S A 111(51):18345-18350. (Cat. #IT-10)
  • Chua JX, Vankemmelbeke M, McIntosh RS, Clarke PA, Moss R, Parsons T, Spendlove I, Zaitoun AM, Madhusudan S, Durrant LG. (2015) Monoclonal Antibodies Targeting LecLex-Related Glycans with Potent Anti-Tumor Activity. Clin Cancer Res 21(13):2963-2974. (Cat. #IT-48)
  • Bostad M, Olsen CE, Peng Q, Berg K, Hogset A, Selbo PK. (2015) Light-controlled endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin by photochemical internalization – A minimally invasive cancer stem cell-targeting strategy. J Control Release 206:37-48. (Cat. #PR-01)
  • Ha KD, Bidlingmaier SM, Zhang Y, Su Y, Liu B. (2014) High-content analysis of antibody phage-display library selection outputs identifies tumor selective macropinocytosis-dependent rapidly internalizing antibodies. Mol Cell Proteomics 13(12):3320-3331. (Cat. #IT-27)
  • Mao CA, Li H, Zhang Z, Kiyama T, Panda S, Hattar S, Ribelayga CP, Mills SL, Wang SW. (2014) T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4/melanopsin-expressing intrinsically photosensitive retinal ganglion cells. J Neurosci 34(39):13083-13095. (Cat. #AB-N39)
  • Kiris E, Wang T, Yanpallewar S, Dorsey SG, Becker J, Bavari S, Palko ME, Coppola V, Tessarollo L. (2014) TrkA in vivo function is negatively regulated by ubiquitination. J Neurosci 34(11):4090-4098. (Cat. #AB-N03)
  • El-Danaf RN, Huberman AD. (2015) Characteristic patterns of dendritic remodeling in early-stage glaucoma: evidence from genetically identified retinal ganglion cell types. J Neurosci 35(6):2329-2343. (Cat. #AB-N39)
  • Peters CM, Hayashida K, Suto T, Houle TT, Aschenbrenner CA, Martin TJ, Eisenach JC. (2015) Individual Differences in Acute Pain-induced Endogenous Analgesia Predict Time to Resolution of Postoperative Pain in the Rat. Anesthesiology 122(4):895-907. (Cat. #IT-03, Cat. #IT-18)
  • Joly S, Guzik-Kornacka A, Schwab ME, Pernet V. (2014) New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci 55(7):4476-4489. (Cat. #AB-N38)
  • Akahoshi N, Kamata S, Kubota M, Hishiki T, Nagahata Y, Matsuura T, Yamazaki C, Yoshida Y, Yamada H, Ishizaki Y, Suematsu M, Kasahara T, Ishii I. (2014) Neutral aminoaciduria in cystathionine beta-synthase-deficient mice; an animal model of homocystinuria. Am J Physiol Renal Physiol 306(12):F1462-76. (Cat. #AB-T036)
  • Ono K, Ye Y, Viet CT, Dang D, Schmidt BL. (2015) TRPV1 expression level in isolectin B4-positive neurons contributes to mouse strain difference in cutaneous thermal nociceptive sensitivity. J Neurophysiol 113(9):3345-3355. (Cat. #IT-10, Cat. #PR-01)
  • Li J, Pan Y, Zhang B, Chen Q. (2015) Macrophages are needed in the progression of tuberculosis into lung cancer. Tumour Biol 36(8):6063-6066. (Cat. #IT-06)
  • Higgins SC, Fillmore HL, Ashkan K, Butt AM, Pilkington GJ. (2015) Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro. Anticancer Res 35(1):77-84. (Cat. #IT-04)
  • Nguyen HN, Huppe-Gourgues F, Vaucher E. (2015) Activation of the mouse primary visual cortex by medial prefrontal subregion stimulation is not mediated by cholinergic basalo-cortical projections. Front Syst Neurosci 9:1. (Cat. #IT-16)
  • Frankel AE, Nymeyer H, Lappi DA, Higgins D, Ahn C, Noe C. (2014) Preliminary results from a phase I study of substance P-saporin in terminal cancer patients with intractable pain. J Clin Oncol 32 (suppl 31):191. (Cat. #IT-07)

Targeting Talk: Product Questions

  • I ordered a targeted toxin. Will it come in powder form? How do I re-dissolve it? [see answer]
  • I’m interested in your anti-DBH-saporin toxin for lesioning central catecholaminergic neurons. I see from the product description that the antibody used is a mouse monoclonal — designed to specifically target rat DBH. My interest is to produce targeted lesions in mouse transgenic. Will this product still work specifically? Thanks. [see answer]
  • Recently we used your flow cytometry services (Cytometry Research, ATS subsidiary). Based on post flow data analysis needs, I am providing the assay group list below (withheld for confidentiality). [see answer]

Drug-free selection of stable transfectants using rIB4-SAP
(continued from page 1)

Upcoming Events

  • Amer Assoc Immunologists / May 8-12, 2015 / New Orleans, LA / Booth #541
  • Society for Neuroscience / October 17-21, 2015 / Chicago, IL / Booth #662

Custom Biotinylation Service

Kermit’s Kollection

Targeting Technology Tutorial

Targeting Teaser (Jumble) – Win a jigsaw puzzle!

Targeting Trends Newsletter 15q1

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page.


The cover article is contributed by this year’s winner of ATS’ SFN Poster of the Year. Aaron Kucinski summarizes his work in Dr. Sarter’s lab using 192-IgG-SAP and Anti-ChAT-SAP to Model Fall Propensity in Parkinson’s Disease. Also, on page 2 is an update on the Veterinary Development of Substance P-Saporin (SP-SAP). ATS is also excited to announce two new products ZAP SrB Development Kit (Cat. #KIT-SrB-Z) and Anti-CD44-SAP (Cat. #IT-72) plus the new Beta Testing Program.

Targeting Talk” answers questions for a lab getting ready to start a project using targeted toxins. The latest articles using ATS products are highlighted in Recent Scientific References and you can solve the “Targeting Teaser” to win a 2015 ATS calendar.

Impairments in gait, posture and complex movement control in rats modeling the multi-system, cholinergic-dopaminergic losses in Parkinson’s Disease
(continued on page 6)

by Aaron Kucinski (Collaborators at University Michigan, Ann Arbor: K. Phillips, R. Albin, M. Sarter)

Related products: 192-IgG-SAP (Cat. #IT-01), Anti-ChAT-SAP (Cat. #IT-42)

Society for Neuroscience Poster of the Year Award

Upcoming Events

  • Amer Assoc Immunologists / May 8-12, 2015 / New Orleans, LA / Booth #541
  • Society for Neuroscience / October 17-21, 2015 / Chicago, IL / Booth #662

Veterinary Development of Substance P-Saporin (SP-SAP)

Recent Scientific References (read summaries)

  • Jokiaho AJ, Donovan CM, Watts AG. (2014) The rate of fall of blood glucose determines the necessity of forebrain-projecting catecholaminergic neurons for male rat sympathoadrenal responses. Diabetes 63(8):2854-2865. (Cat. #IT-03, Cat. #IT-18)
  • Ehrlich D, Wang B, Lu W, Dowling P, Yuan R. (2014) Intratumoral anti-HuD immunotoxin therapy for small cell lung cancer and neuroblastoma. J Hematol Oncol 7(1):91. (Cat. #IT-27)
  • Nichols NL, Vinit S, Bauernschmidt L, Mitchell GS. (2015) Respiratory function after selective respiratory motor neuron death from intrapleural CTB-saporin injections. Exp Neurol 267:18-29 (Cat. #IT-14, Cat. #PR-01)
  • Vazquez-DeRose J, Schwartz MD, Nguyen AT, Warrier DR, Gulati S, Mathew TK, Neylan TC, Kilduff TS. (2016) Hypocretin/orexin antagonism enhances sleep-related adenosine and GABA neurotransmission in rat basal forebrain. Brain Struct Funct 221(2):923-940. (Cat. #IT-01)
  • Sims S, Klenerman P. (2015) Increasing inflationary T-cell responses following transient depletion of MCMV-specific memory T cells. Eur J Immunol 45(1):113-118. (Cat. #IT-27)
  • Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S. (2014) A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells. Biotechnol J Epub (Cat. #IT-10)
  • Kalinchuk AV, Porkka-Heiskanen T, McCarley RW, Basheer R. (2015) Cholinergic neurons of the basal forebrain mediate biochemical and electrophysiological mechanisms underlying sleep homeostasis. Eur J Neurosci 41(2):182-195. (Cat. #IT-01)
  • Speer CM, Sun C, Liets LC, Stafford BK, Chapman B, Cheng HJ. (2014) Eye-specific retinogeniculate segregation proceeds normally following disruption of patterned spontaneous retinal activity. Neural Dev 9(1):25. (Custom Conjugate)
  • Akiyama T, Tominaga M, Takamori K, Carstens MI, Carstens E. (2014) Role of spinal bombesin-responsive neurons in nonhistaminergic itch. J Neurophysiol 112(9):2283-2289. (Cat. #IT-40)
  • Mang Y, Zhao Z, Zeng Z, Wu X, Li Z, Zhang L. (2015) Efficient elimination of CD103-expressing cells by anti-CD103 antibody drug conjugates in immunocompetent mice. Int Immunopharmacol 24(1):119-127. (Custom Conjugate)
  • Jeong da U, Lee JE, Lee SE, Chang WS, Kim SJ, Chang JW. (2014) Improvements in memory after medial septum stimulation are associated with changes in hippocampal cholinergic activity and neurogenesis. Biomed Res Int 2014:568587. (Cat. #IT-01)
  • Feetham CH, Barrett-Jolley R. (2014) NK1-receptor-expressing paraventricular nucleus neurones modulate daily variation in heart rate and stress-induced changes in heart rate variability. PHY2 2(12):e12207. (Cat. #IT-11)
  • Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S. (2013) Targeted toxin-based selectable drug-free enrichment of Mammalian cells with high transgene expression. Biology (Basel) 2(1):341-355. (Cat. #IT-10)

Targeting Talk: Product Questions

  • Our lab is getting ready to begin a project using one of your targeted toxins. We already did a preliminary experiment to try out the material, but we have a couple of questions before we start the larger project. First, do you have any protocols or references for injecting intrathecally? [see answer]
  • The second question is in two parts: 1) how do we determine the appropriate dose, and 2) how do we know saporin is not killing indiscriminately at that dose? [see answer]

IgG Quantification by ELISA

Modeling Fall Propensity in Parkinson’s Disease
(continued from page 1)

Targeting Teaser Winners

ZAP SrB Development Kit (Cat. #KIT-SrB-Z)

Anti-CD44-SAP (Cat. #IT-72)

Beta Testing Program

Kermit’s Kollection

Targeting Technology Tutorial

Targeting Teaser (Jumble) – Win a 2015 ATS calendar!