Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfuntion of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer’s disease, Down and […]

Li J, Rao D, & Gibbs RB. Effects of Cholinergic Lesions and Cholinesterase Inhibitors on Aromatase and Estrogen Receptor Expression in Different Regions of the Rat Brain. (2018). Neuroscience, 384 203-213. Dose:  Rats received intraseptal injections of 2.0 ml (0.2 mg/ml) or icv injections of 0.4 mcg.  Lesions resulted in loss of ChAT-positive cells in the septum, and

Targeting Results from FENS (The Federation of European Neuroscience Societies) will be presented at this year’s meeting in Berlin. Advanced Targeting Systems congratulates the researchers on their work and looks forward to viewing the posters and interacting with scientists from around the world at this year’s meeting in Berlin. Come visit us at Booth 165

Gene therapy has been proposed for a variety of genetic disorders, including severe combined immunodeficiency, but it has not yet found routine use in the clinic, in part because of potential complications. To help pave the way for safer translation of such gene therapy, Schiroli et al. studied potential approaches to gene therapy in mouse

Enhanced Targeting of Triple-Negative Breast Carcinoma and Malignant Melanoma by Photochemical Internalization of CSPG4-Targeting Immunotoxins. Eng MS, Kaur J, Prasmickaite L, Engesaeter BO, Weyergang A, Skarpen E, Berg K, Rosenblum MG, Maelandsmo GM, Hogset A, Ferrone S, & Selbo PK. (2018). Photochemical & Photobiological Sciences 17(5):539-551. Conclusion: The combination of the drug delivery technology PCI

Effects of Cholinergic Lesions and Cholinesterase Inhibitors on Aromatase and Estrogen Receptor Expression in Different Regions of the Rat Brain. Li J, Rao D, & Gibbs RB. Neuroscience Available online 29 May 2018 In Press, Uncorrected Proof. Highlights This paper applied a novel UPLC–MS/MS method to detect aromatase (ARO) activity in the brain tissue. Medial

Agents of Interest in Pain Research Agent Target Lesioning Use 192-IgG-SAPCat. #IT-01 p75NTR, low affinity NGFR(cholinergic basal forebrain, cerebellar Purkinje neurons,postganglionic autonomic neuronsand some primary sensory neurons) Immunolesioning Anti-DBH-SAPCat. #IT-03 Dopamine beta hydroxylase(adrenergic and noradrenergic neurons) Immunolesioning by applicationto either dendrites/soma orby retrograde axonal transport Anti-SERT-SAPCat. #IT-23 Sertonin transporter(serotonergic neurons) Immunolesioning Dermorphin-SAPCat. #IT-12 mu opioid receptor(striatal

In a study produced by Ferrini et al. indicates that morphine-induced hyperalgesia, as neuropathic pain, is a pathological alteration of pain sensitivity whose expression is gated by spinal microglia. Targeting at any level, this microglia-to-neuron cascade is a valuable strategy to improve the use of morphine in chronic pain. Figure at right: Microglia depletion in

The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones. Watanabe S, Sakurai T, Nakamura S, Miyoshi K, & Sato M. (2018). Int J Mol Sci, 19 (4). Objective:  Most genome editing systems employ transient treatment with selective drugs such as puromycin to obtain the desired genome-edited