Targeting ChAT – An innovative tool to Study Alzheimer’s Disease

Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfuntion of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer’s disease, Down and Rett syndromes.

Anti-ChAT-SAP (Cat. #IT-42) is made with an antibody using a 22-amino acid peptide from porcine ChAT (GLF SSY RLP GHT QDT LVA QKSS). The targeted toxin recognizes porcine ChAT and is expected to cross-react with rat, mouse, and human.

Representative coronal sections of the rat striatum show intact nuclei (NeuN) staining but clear ablation of the cholinergic interneurons with ChAT staining in lesioned cases (DMS or VS). Abbreviations; DMS: dorsomedial striatum, VS: ventral striatum, LV: lateral ventricle, CC: corpus callosum, AC, anterior commissure. Scale bar: 1 mm. Reprinted from Aoki et al. (2015).

Anti-ChAT-SAP References