Li J, Rao D, & Gibbs RB. Effects of Cholinergic Lesions and Cholinesterase Inhibitors on Aromatase and Estrogen Receptor Expression in Different Regions of the Rat Brain. (2018). Neuroscience, 384 203-213.
Dose: Rats received intraseptal injections of 2.0 ml (0.2 mg/ml) or icv injections of 0.4 mcg. Lesions resulted in loss of ChAT-positive cells in the septum, and ChAT activity in the hippocampus. Septal infusions eliminated most of the ChAT-IR cells in MS; ChAT activity in hippocampus also significantly decreased. ChAT activity in the frontal cortex was not significantly affected.
Objective: To determine if effects of cholinergic inputs on synaptic plasticity and neuronal function are mediated by effects on local estrogen production or ER expression.
Summary: Selectively destroying cholinergic projections to the hippocampus had little effect on ARO and ER expression in many regions of the rat brain.