targeted-toxins

Ballmaier M, Casamenti F, Scali C, Mazzoncini R, Zoli M, Pepeu G, Spano PF (2002) Rivastigmine antagonizes deficits in prepulse inhibition induced by selective immunolesioning of cholinergic neurons in nucleus basalis magnocellularis. Neuroscience 114(1):91-98. doi: 10.1016/s0306-4522(02)00234-8

Summary: The authors injected 300 nl of 400 ng/ml 192-Saporin (Cat. #IT-01) bilaterally into the nucleus basalis magnocellularis of rats, then treated the lesioned animals with rivastigmine, a cholinesterase inhibitor. Animals treated with rivistagmine exhibited raised levels of cortical acetylcholine, in contrast to undetectable acetylcholine levels in lesioned animals not treated with rivastigmine.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Cassel J, Gaurivaud M, Lazarus C, Bertrand F, Galani R, Jeltsch H (2002) Grafts of fetal septal cells after cholinergic immunotoxic denervation of the hippocampus: a functional dissociation between dorsal and ventral implantation sites. Neuroscience 113(4):871-882. doi: 10.1016/s0306-4522(02)00226-9

Summary: The authors lesioned rats with intraseptal infusions of 0.8 µg 192-Saporin (Cat. #IT-01), then implanted fetal cells in either the dorsal or ventral hippocampus. Only grafts into the dorsal hippocampus counteracted the effect of cholinergic lesions on spatial working memory performance.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Pizzo DP, Thal LJ, Winkler J (2002) Mnemonic deficits in animals depend upon the degree of cholinergic deficit and task complexity. Exp Neurol 177:292-305. doi: 10.1006/exnr.2002.7993

Summary: In this study, the authors compared icv and intraparenchymal injections of 192-Saporin (Cat. #IT-01, 3.3 µg and 450 ng, respectively). While a similar reduction in choline acetyltransferase activity was observed with each strategy, and performance in certain allocentric tasks was similar, an egocentric task showed a marked difference between the two groups.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Vale-Martinez A, Baxter MG, Eichenbaum H (2002) Selective lesions of basal forebrain cholinergic neurons produce anterograde and retrograde deficits in a social transmission of food preference task in rats. Eur J Neurosci 16(6):983-998. doi: 10.1046/j.1460-9568.2002.02153.x

Summary: Injections of 0.2 µg 192-Saporin (Cat. #IT-01) were made into either the medial septum/vertical limb of the diagonal band (MS/VDB), or the nucleus basalis magnocellularis/substantia innominata (NBM/SI) of rats. MS/VDB lesions had no effect on anterograde memory, while NBM/SI lesions strongly impaired immediate and 24-hour retention. In contrast, MS/DVB lesions produced significant memory deficits in a long-delay retrograde memory test.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Nattie EE, Li A (2002) Substance P-saporin lesion of neurons with NK1 receptors in one chemoreceptor site in rats decreases ventilation and chemosensitivity. J Physiol 544(Pt 2):603-616. doi: 10.1113/jphysiol.2002.020032

Summary: The authors injected 0.1 pmol SP-SAP (Cat. #IT-07) into the retrotrapezoid nucleus/parapyramidal region of rats. The lesioned animals demonstrated hypoventilation while at rest, decreased response to high CO2 levels, and a tendency to sleep less.

Related Products: SP-SAP (Cat. #IT-07)

Truitt WA, Coolen LM (2002) Identification of a potential ejaculation generator in the spinal cord. Science 297(5586):1566-1569. doi: 10.1126/science.1073885

Summary: The authors lesioned a specific population of rat spinothalamic neurons using 6-8 injections of 4 ng SSP-SAP (Cat. #IT-11). Whereas the treated rats exhibited no change in sexual behavior such as mounts and intromissions, ejaculatory behavior was completely abolished. The data suggest that this population of neurons may function as an ejaculation generator in the spinal cord.

Related Products: SSP-SAP (Cat. #IT-11)

Burgess SE, Gardell LR, Ossipov MH, Malan T, Vanderah TW, Lai J, Porreca F (2002) Descending facilitation from the rostral ventromedial medulla maintains, but does not initiate, neuropathic pain. IASP 2002 Abstracts International Association for the Study of Pain, San Diego, CA.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Arunkumar R, Ackerman LL, Jones III R, Holdsworth R, Proudfit HK, Hammond DL (2002) Loss of IB4 staining in dorsal root ganglion neurons after spinal nerve ligation is not the result of cell death. IASP 2002 Abstracts International Association for the Study of Pain, San Diego, CA.

Related Products: IB4-SAP (Cat. #IT-10)

McGaugh JL (2002) Memory consolidation and the amygdala: A systems perspective. Trends Neurosci 25(9):456-461. doi: 10.1016/s0166-2236(02)02211-7

Objective: To investigate the involvement of basolateral region of the amygdala (BLA) projections.

Summary: The BLA acts via projections to brain regions involved in forming and consolidating memories of different kinds of experiences.

Usage: Lesions of nucleus basalis magnocellularis (NBM) induced by infusions of 192 IgG-SAP block the memory enhancement (in a 48-hr retention test), as induced by post-training infusions of noradrenaline administered into the basolateral amygdala immediately after inhibitory avoidance training.

Related Products: 192-IgG-SAP (Cat. #IT-01)

See Also:

• Power AE et al. Lesions of the nucleus basalis magnocellularis induced by 192 IgG-saporin block memory enhancement with posttraining norepinephrine in the basolateral amygdala. Proc Natl Acad Sci U S A 99(4):2315-2319, 2002.

Tarpley JW, MacIntyre E, Martin WJ (2002) Loss of IB4-positive sensory neurons mitigates the consequences of nerve injury in the rat. IASP 2002 Abstracts International Association for the Study of Pain, San Diego, CA.

Related Products: IB4-SAP (Cat. #IT-10)