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Steroid sulfatse inhibition potentiates working memory deficit induced by 192 IgG-saporin

Zambon NJ, Liberatore MA, Nagle RA, Pokala VN, Li P, Johnson DA (2002) Steroid sulfatse inhibition potentiates working memory deficit induced by 192 IgG-saporin. Neuroscience 2002 Abstracts 82.6. Society for Neuroscience, Orlando, FL.

Summary: Infusion of 192 IgG-saporin (SAP) into the medial septum (MS) of rats selectively lesions cholinergic neurons and impairs acquisition of a delayed matching to position T-maze task. Since steroid sulfatase inhibitors allosterically inhibit the GABA-A receptor and enhance cognition, the present study investigated the cognitive effects of sulfatase inhibition on SAP lesioned animals. Male Sprague-Dawley rats received intraseptal infusions of either cerebrospinal fluid (CSF) or 0.22 μg/μl SAP. Eight days after the infusion, the rats were given an IP injection of either the steroid sulfatase inhibitor DU-14 (30mg/ml) or vehicle daily for 14 days, then every other day during the testing period. In the acquisition phase of testing, each rat completed 8 trial pairs per day until reaching criterion (15 of 16 correct choices). The rats were then tested for retention of the task by inserting delays of 10, 20, and 30 seconds after the first trial of a pair. DU-14 potentiated the impairment in acquisition produced by SAP. The introduction of a delay resulted in deceased performance in all treatment groups except rats administered DU-14 without SAP lesioning. These results suggest that DU-14 may enhance cognition in cholinergically intact animals, but the combination of DU-14, with cholinergic lesioning of MS, impairs working memory by inhibition of both cholinergic and GABAergic neurotransmission.

Related Products: 192-IgG-SAP (Cat. #IT-01)

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