Pokala VN, Libertore MA, Zambon NJ, Nagle RA, Li PK, Witt-Enderby PA, Johnson DA (2002) Effect of 192 IgG-saporin medial-septum lesion on hippocampal receptor density in rats. Neuroscience 2002 Abstracts 82.7. Society for Neuroscience, Orlando, FL.
Summary: The aim of the study was to quantify the effect of selective cholinergic neurotoxin192 IgG-saporin (SAP) medial septal (MS), lesions on hippocampal muscarinic, GABAA and NMDA receptor density. Rats were injected with SAP (0.22 and 0.45 μg/μl) into the MS. After 6 weeks, hippocampal tissue was collected and saturation radioligand binding assays were performed to determine the receptor density. 3H-QNB was a muscarinic antagonist, 3H-muscimol a GABAergic agonist and 3H-MK-801 an NMDA antagonist were utilized. The results demonstrated a dose dependent increase in muscarinic receptor density (B-max) of 226 and 355% respectively. These results suggest changes in receptor density in response to hippocampal cholinergic denervation.
Related Products: 192-IgG-SAP (Cat. #IT-01)