References

Ahn JP (2022) Ablation of dorsomedial striatum patch compartment results in modification to reward-driven behaviors in rats. Mercer University School of Medicine Thesis.

Objective: This thesis intended to demonstrate that selective ablation of dorsomedial striatum (DMS) patch compartment neurons results in a significant impact on the initial development of reward-driven behaviors during the early stages of drug seeking behavior.

Summary: Through the use Dermorphin-SAP and training rats to self-administer cocaine, ablation of the patch compartment of the DMS resulted in an increase in early-stage lever pressing, suggesting that the DMS patch compartment contributes to reward-driven behaviors.

Usage: 17 ng/µl Dermorphin-SAP in sterile artificial cerebrospinal fluid (aCSF) to selectively ablate patch compartment neurons. Infusions into either the dorsomedial striatum or dorsolateral striatum (2 µl of infusion liquid).

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Bosecke C (2022) A novel anxiety process biomarker via electrovestibulography. University of Manitoba Thesis.

Objective: Use electrophysiological technique of Electrovestibulography to measure field potentials caused by vestibular neurons in the ear canal in order to identify biomarkers associated with anxiety disorder.

Summary: Anxiety disorders have no known biomarkers and are diagnosed based on symptoms. Identifying biomarkers could help improve the accuracy of anxiety disorder diagnosis, but can be difficult because the brain regions implicated in anxiety are very deep within the brain.

Usage: 192 IgG-SAP (Cat. #IT-01) was used to lesion the cholinergic medial septum inputs to the hippocampus. 192 IgG-saporin was diluted to 0.35 µg/µl with sterile saline, and 0.4 µl was infused bilaterally into each ventral site of the medial septum.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Copeland EN (2022) Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice. Brock University St. Catharines, Ontario, Canada Thesis. PMID: 0

Objective: To investigate the differences between two models of Duchenne muscular dystrophy (DMD): D2 mouse model with young mice showing cognitive deficits, and C57, the traditional mouse model.

Summary: Impaired sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function in both C57 and D2 mdx brain – perhaps due to heightened oxidative/nitrosative stress.

Usage: Western blot; NO-L-Cysteine Mouse Monoclonal, Conjugated as a marker of oxidative/nitrosative stress.

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Li Y, Ye Z, Yang H, Xu Q (2022) Tailoring combinatorial lipid nanoparticles for intracellular delivery of nucleic acids, proteins, and drugs. Acta Pharm Sin B 12(6):2624-2639. doi: 10.1016/j.apsb.2022.04.013

Objective: To highlight the recent progress in combinatorial lipid nanoparticles (LNPs) with novel structures and properties for the delivery of small- and macromolecular therapeutics.

Summary: The administration of protein/LNP negatively impacted reproduction in rats, including sperm production, estrous cyclicity and testicular and ovarian morphology, without causing any significant side effects. This non-surgical approach can be developed into a safe and convenient strategy for controlling the overproduction of pet and wildlife.

Usage: Intravenous administration of saporin loaded LNPs

Related Products: Saporin (Cat. #PR-01)

Chen LQ, Lv XJ, Guo QH, Lv SS, Lv N, Yu J, Xu WD, Zhang YQ (2022) Asymmetric activation of microglia in the hippocampus drives anxiodepressive consequences of trigeminal neuralgia. bioRxiv 2022.04.16.488241. doi: 10.1101/2022.04.16.488241

Related Products: Mac-1-SAP rat (Cat. #IT-33)

Sakarin S, Surachtpong SD, Rungsipipat A (2022) Expression of proteins related to serotonin signaling in the lungs of dogs affected with pulmonary hypertension secondary to degenerative mitral valve disease. Thai J Vet Med 52(suppl.):31-33. doi: 10.5555/20220315534

Objective: To detect the expression of proteins related to the serotonin pathway in the lung tissue of dogs affected with pulmonary hypertension (PH) secondary to degenerative mitral valve disease (DMVD) compared to DMVD and healthy control dogs.

Summary: Proteins related to the serotonin signaling pathway were increased in lung tissue of DMVD dogs with PH, suggesting that the serotonin signaling pathway may be involved with the pathogenesis of PH. Inhibition of proteins related to the serotonin signaling pathway may be a target of interest or provide a novel therapeutic approach in PH secondary to DMVD.

Usage: SERT Mouse Monoclonal (Cat. #AB-N40) Immunohistochemistry (1:200).

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Mueller M, Thompson R, Osman KL, Andel E, DeJonge CA, Kington S, Stephenson Z, Hamad A, Bunyak F, Nichols NL, Lever TE (2022) Impact of limb phenotype on tongue denervation atrophy, dysphagia penetrance, and survival time in a mouse model of ALS. Dysphagia 37(6):1777-1795. doi: 10.1007/s00455-022-10442-4 PMID: 35426522

Related Products: CTB-SAP (Cat. #IT-14)

See Also:

• Lind LA et al. Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection. Muscle Nerve 63(3):413-420, 2021.

Marcucci F, Lappi DA, Ghislieri M, Martineau D, Siena S, Bregni M, Soria M, Gianni AM (1989) In vivo effects in mice of an anti-T cell immunotoxin. J Immunol 142:2955-2960.

Related Products: OX7-SAP (Cat. #IT-02)

Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807

Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.

Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.

Usage: Cell Sorting (1:400)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778 PMID: 35453462

Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.

Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)