tt2002

Targeting Trends 2002 Newsletter

2002, issue 1: Control Conjugates: The Perfect Companion for Targeted Toxins / by Lappi DA / featuring Control Conjugates SFN Poster of the Year Targeting Talk: Toxin Safety Targeting Tools: Serotonin Transporter Antibody 2002, issue 2: HCRT-SAP Lesion Produces Sleepiness While anti-DBH-SAP Lesion Does Not  / by Blanco-Centurion C / featuring Anti-DBH-SAP (Cat. #IT-03) Targeting Talk: …

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Targeting Topics 02q4

Unilateral lesions of the cholinergic basal forebrain and fornix in one hemisphere and inferior temporal cortex in the opposite hemisphere produce severe learning impairments in rhesus monkeys. Easton A, Ridley RM, Baker HF, Gaffan D. Cereb Cortex 12(7):729-736, 2002. PMID: 12050084 The authors used a combination of basal forebrain lesioning using ME20.4-SAP (Cat. #IT-15) and …

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Cover Article: Does 192-IgG-Saporin or 5,7-DHT kill cognitive functions in the rat?

Contributed by Jean-Christophe Cassel, PhD, Laboratoire de Neurosciences Cognitives et Comportementales, UMR 7521 Université Louis Pasteur/CNRS, Strasbourg, France & Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Universität Freiburg, Neuropharmakologisches Labor, Freiburg, Germany. Dr. Cassel and colleagues are the authors of four publications released this quarter using 192-Saporin (Cat. #IT-01) Trying to understand how …

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Targeting Talk: Neuropeptide Toxins

What are neuropeptide-toxins and how do they work? Neuropeptide-toxin conjugates are made up of the ribosome-inactivating protein, saporin, coupled to a naturally-occurring or synthetically-modified neuropeptide such as Substance P or dermorphin. The conjugate has binding specificity similar to the native, unconjugated neuropeptide. When the neuropeptide binds to its cognate receptor, the conjugate is internalized. Once …

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Targeting Topics 02q3

Impairments in negative patterning, but not simple discrimination learning, in rats with 192 IgG-saporin lesions of the nucleus basalis magnocellularis. Butt AE, Noble MM, Rogers JL, Rea TE. Behav Neurosci 116(2):241-255, 2002. PMID: 11996310 192-Saporin (Cat. #IT-01) administration to the basal forebrain has frequently been used in rats to create a model for Alzheimer’s disease. …

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Cover Article: Saporin Immunotoxins for Treating Human Hematological Malignancies

Contributed by David J Flavell, PhD, The Simon Flavell Leukaemia Research Unit, Division of Cancer Sciences, University of Southampton Medical School, United Kingdom. I have asked Dr. Flavell to contribute an article on the clinical use of his saporin immunotoxins. Dr. Flavell’s research unit is named after his son, who died from leukemia. At that …

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Targeting Tools: OX7 and OX7-SAP

OX7-SAP is a cytotoxin to many types of neurons. It is one of the most potent of the saporin immunotoxins— the conjugates between a cell surface antibody and the ribosome-inactivating protein from Saponaria officinalis. The reason for the potency could be due to the construct’s grand tradition. The antibody, OX7, is one of the original …

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Targeting Talk: Toxin Safety

Q: Your recent issue of Targeting Trends stated that it was unlikely that saporin compounds or constituents would be excreted in urine or feces. However, you acknowledge that experimental data is lacking. Have there been any tests of animal urine or feces for saporin content? My animal care staff are concerned. A: One of the …

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