Q: I have a question about Retrograde Transport. There is a comment in the FAQ “In fact, we recommend that you wait two weeks at least to see immunohistological evidence of a toxic effect after injection of a saporin toxin in vivo.” Are there data to support this recommendation? As a researcher who utilizes your […]
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Contributed by Dr. Naoki Yoshimura, Dept Urology/Pharmacology, Univ Pittsburgh School of Medicine, Pittsburgh PA 15213.Dr. Yoshimura summarizes his work with IB4-SAP. A complete report was published in Eur J Neurosci 20(2):474-482, 2004. It has been demonstrated that hyperexcitability of C-fibers in bladder afferent pathways can contribute to bladder overactivity and/or bladder pain under pathological conditions
Cover Article: Effects of IB4-SAP on Bladder Overactivity Read More »
Advanced Targeting Systems’ technology – Molecular Surgery – is a modification of one of the most widely used techniques in the neurosciences: lesioning of a region by surgical means and observation of the effect. Saporin has no way of entering a cell on its own, except by bulk-phase endocytosis. To be effective, Saporin is bound
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Selective Cholinergic Depletion of Pedunculopontine Tegmental Nucleus Aggravates Freezing of Gait in Parkinsonian Rats. Xiao, H, Li, M, Cai, J, et al. (2017). Neurosci Lett [Epub 10 August 2017]. PMID: 28803956 Many patients with advanced Parkinson’s disease suffer from gait and postural impairments. The authors used Anti-ChAT-SAP (Cat. #IT-42) to specifically lesion neurons in the Pedunculopontine Tegmental Nucleus (PPTg) to examine the
Parkinson’s Disease, Unsteady Gait, and the ChAT Walk Read More »
Isolectin B4 (IB4) is a tetrameric protein isolated from Griffonia simplicifolia seeds. Although one of its first uses was the agglutination of B-type red blood cells,[1] it has also been used extensively in the neurosciences because the alpha-galactosyl residues bound by IB4 are present on a variety of cell populations such as the non-peptidergic afferents
Targeting Tools: Recombinant Isolectin B4 (IB4) Read More »
Q: Are all lots of 192-Saporin (192-IgG-SAP, Cat. #IT-01) the same? A: There are variances from lot to lot, and Advanced Targeting Systems includes a cytotoxicity graph on the data sheet with each product comparing the current lot with previous lots. Chemicon International also sells immunotoxins, and used to distribute ATS products. However, since early
Not the Right Stuff Read More »
Contributed by Lisa E. Goehler and Ronald P.A. Gaykema Center for the Study of Complementary and Alternative Therapies, University of Virginia School of Nursing, Charlottesville, VA 22908 Fatigue, experienced as reduced motivation and motor activity, is common in acute and chronic disease, including heart disease, cancer and cancer chemotherapy. Fatigue is part of a constellation
GAT-1 is a sodium-coupled neurotransmitter transporter responsible for moving g-aminobutyric acid (GABA) across cell membranes. GABA is the predominant inhibitory neurotransmitter in the mammalian central nervous system. GAT-1 is widely distributed in both the central and peripheral nervous systems. GAT-1 and GABA are present in numerous neuronal pathways, some of which are implicated in epilepsy,
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In a study produced by Ferrini et al. indicates that morphine-induced hyperalgesia, as neuropathic pain, is a pathological alteration of pain sensitivity whose expression is gated by spinal microglia. Targeting at any level, this microglia-to-neuron cascade is a valuable strategy to improve the use of morphine in chronic pain. Ferrini F, Trang T, Mattioli TA,
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ATS has been awarded a Phase I SBIR grant to develop a new line of products. Dr. Douglas Lappi is the Principal Investigator and Brian Russell is the Lead Scientist on the project. Molecules targeted towards cell surface markers have been used for years to identify specific cell types. It has been demonstrated over the
Targeting Article: SBIR Grant Funded: Selective Activation in Neuronal Populations Read More »