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Tools to study neuroplasticity induced by chronic use of opioids

SSP-SAP [Stable analog of Substance P conjugated to Saporin] (Cat. #IT-11) Objective:  To determine the the mechanisms mediating the induction of opioid-induced hyperalgesia and the prolongation of prostaglandinE2-induced hyperalgesia in type II hyperalgesic priming. Summary:  The authors show that understanding the mechanisms responsible for the induction of type II hyperalgesic priming, a form of neuroplasticity in the […]

Catecholamine neurons in Obesity

Anti-DBH-SAP [Dopamine Beta Hydroxylaxe antibody conjugated to Saporin] (Cat. #IT-03) Objective: To investigate the role of hindbrain catecholamine neuron pathways and their contribution to long-term energy homeostasis by controlling obesogenic sensitivity to a high-fat, high sucrose choice diet. Summary: The authors show that catecholamine neurons (primarily in the VLM and NTS) convey essential feedback signals […]

Streptavidin-Saporin key ingredient in Immunotoxin Cocktail

Streptavidin-ZAP [Streptavidin conjugated to Saporin] (Cat. #IT-27) Objective: To determine if an immunotoxin cocktail targeted to multiple epitopes has synergistic effects on low expression level cells, which would expand the applicable range of immunotoxin therapy for cancer. Summary: The combination of immunotoxins with different mechanisms of action in an antibody cocktail will increase cytotoxic activities […]

A highly specific NGFR antibody

NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP) Objective: To determine if differentiated neural crest cells are promising supporting cell candidates to aid in peripheral nerve repair. Summary: In this study, neural crest cells were differentiated from human embryonic stem cells. NGFR (mouse monoclonal, 1:100) Immunocytochemistry.  The specificity of NGFR antibody was validated by immunocytochemical […]

Strategies to overcome 5-FU resistance in cancer

Congratulations to the Selbo laboratory for their recent publication using Anti-CD105-SAP. This targeted toxin was previously part of the BETA program. As first to publish using this product, Selbo’s group receives a $500 product credit and applause for their great work. 5-Fu Resistant Emt-Like Pancreatic Cancer Cells Are Hypersensitive to Photochemical Internalization of the Novel […]

Screening Aptamers as Targeting Agents to Deliver Payloads

Aptamers are small, synthetic, single-stranded nucleic acids that fold into unique secondary structures. Unlike peptides and monoclonal antibodies, they are essentially non-immunogenic even when administered in excess amounts. The publications below explain how biotinylated aptamers combined with Streptavidin-conjugated saporin (Streptavidin-ZAP) can be used to screen aptamers for their ability to internalize into a specific cell […]

Streptavidin-ZAP evaluates antibodies for targeted tumor treatment

Characterization of the First Fully Human Anti-Tem1 Scfv in Models of Solid Tumor Imaging and Immunotoxin-Based Therapy. Yuan, X, Yang, M, Chen, X, et al. (2018). Cancer Immunol Immunother 2018/01/10. Streptavidin-ZAP (Cat. #IT-27) Objective: ScFv78 was conjugated with the ribosome-inactivating protein saporin (Streptavidin-ZAP) to evaluate whether scFv78 may be used as a vehicle for theTEM1-targeted […]

New publications: Melanopsin, Serotonin, Streptavidin

2018 starts off with 3 new publications using ATS products. Check out these articles and take advantage of special promotions for each of the products used. Melanopsin Retinal Ganglion Cells Are Not Labeled in Thy-1yfp-16 Transgenic Mice. Grillo, SL, & Stella, SL, Jr. (2018). Neuroreport, 29 (2):118-122. 2017/12/19. Objective: to determine whether mRGCs are labeled […]

IB4-SAP to study mechanical hyperalgesia

To determine which subtype of nociceptor, peptidergic or non-peptidergic, is involved in the mechanical hyperalgesia induced by LMWH (low molecular weight hyaluronan), rats were pretreated with neurotoxins previously shown to selectively deplete these two types of sensory neurons:  Isolectin B4 (IB4)-saporin (SAP), which acts on IB4-positive, non-peptidergic neurons (Cat. #IT-10 IB4-SAP), and substance P-saporin (Cat. […]

Neuromedin B and Itch

To examine the role of NMBR+ dorsal horn neurons in itch and pain transmission, the authors treated mice with NMB-SAP (Cat. #IT-70; 2–3 μg, i.t.) at which no side effects were observed. NMB-SAP treated mice barely showed scratching behaviors, whereas control mice exhibited NMB-induced scratching with a rapid onset of scratching responses. These data demonstrate […]