Top Five Targeted Toxins #1. CTB-SAP (Cat. #IT-14) – targets cells expressing GM1 receptor #2. Anti-DBH-SAP (Cat. #IT-03) – targets cells expressing rat dopamine beta-hydroxylase #3. IB4-SAP (Cat. #IT-10) – targets cells expressing alpha-D-galactopyranoside residues #4. mu p75-SAP (Cat. #IT-16) – targets cells expressing mouse p75NTR #5. ME20.4-SAP (Cat. #IT-15) – targets cells expressing multiple mammalian […]
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Read the complete 2020 Review including recent scientific references.
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Q: We recently spoke to you about performing a custom saporin conjugation using our antibody. Is 0.09% azide in PBS in the antibody stock acceptable? A: There are a number of dialysis steps within the conjugation protocol that will ultimately remove the azide from your antibody solution. So as long as your antibody will be
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Kohno K, Shirasaka R, Yoshihara K, Mikuriya S, Tanaka K, Takanami K, Inoue K, Sakamoto H, Ohkawa Y, Masuda T, Tsuda M (2022) A spinal microglia population involved in remitting and relapsing neuropathic pain. Science 376(6588):86-90. doi: 10.1126/science.abf6805 Objective: To investigate pain recovery mechanisms. Summary: The authors reveal a mechanism for the remission and recurrence
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Dedoni S, Olianas A, Manconi B, Collu M, Tuveri B, Vincis ME, Olianas MC, Onali P (2022) Upregulation of p75NTR by histone deacetylase inhibitors sensitizes human neuroblastoma cells to targeted immunotoxin-induced apoptosis. Int J Mol Sci 23(7):3849. doi: 10.3390/ijms23073849 PMID: 35409209 Summary: Histone deacetylase (HDAC) inhibitors have been useful chemotherapy agents in the treatment of
Q: Is the dosage of Fab-ZAP always enough for any level of antibody concentration? A: The 4.5 nM dosage of Fab-ZAP is the recommended concentration. We do not typically see unspecific killing at 4.5 nM on most cell lines. If the concentration of Fab-ZAP is increased, it may undergo bulk-phase endocytosis and kill cells indiscriminately.
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Q: Is there a recommended ratio between Fab-ZAP dosage and antibody concentration? A: A recommended good starting point is 4.5 nM of Fab-ZAP, with a titration of the antibody starting at a concentration of 10 nM. Related Products: ZAP Conjugates
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Q: Each concentration is suggested to perform 6 replications, can it be adjusted more or less in practice? A: Yes, the assay design is meant to be a thorough approach but can be adjusted by the user. We recommend 6 replications based on our 96-well plate template design. The concentration of Fab-ZAP is 4.5 nM
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Q: Can Fab-ZAP detect the targeted antibody still in supernatant? A: As long as there is nothing in the supernatant inhibiting the reactivity of Fab-ZAP, it should work. We do not typically recommend this, but in theory it should be possible. I would be cautious of this approach based off of the presumed lack of
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Q: Instead of performing the reaction between our biotinylated peptide and Streptavidin-ZAP at the initially provided concentration of Strep-ZAP (20 µM), is it OK if the reaction is done at a 10-fold more dilute concentration? This request is to ensure we don’t have any solubility problems with our very tricky lipophilic peptide. Our protocol would
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