Question: Where does the saporin payload release after internalization? For example, does it require trafficking into a late endosome/lysosomal compartment? Answer: Thank you for reaching out to us. Hopefully I can help answer some of your questions regarding what happens to saporin after being internalized. I would first like to refer you to an article we […]
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Keilholz A, Osman K, Smith C, Streeter K, Ozden I, Lever T, Nichols N (2024) Tongue exercise-induced plasticity in upper airway EMG activity in a model of hypoglossal motor neuron loss. Am Physiol Summit 39(S1) doi: 10.1152/physiol.2024.39.S1.1405 Objective: To study the impact of XII LMN degeneration and develop therapies to improve swallowing and breathing function/coordination.
Ketabforoush A, Wang M, Smith C, Arnold WD, Nichols N (2024) Longitudinal assessment of outcome measures of diaphragm motor unit connectivity as biomarkers of phrenic motor unit degeneration and compensation. Am Physiol Summit 39(S1) doi: 10.1152/physiol.2024.39.S1.1402 Objective: Using CTB-SAP to lesion motor neurons and measuring motor neuron connectivity and cell death, as a model for
Lewis R, Smith C, Nichols N (2024) Spinal TNF-α receptor expression in a rodent model of respiratory motor neuron death. Am Physiol Summit 39(S1) doi: 10.1152/physiol.2024.39.S1.1401 Objective: To create a model from respiratory deficit and study the role of inflammation in phrenic long-term facilitation. Summary: Some neurodegenerative diseases are characterized by deficits in respiratory neurons,
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Toledo C, Del Rio R (2024) Cardiorespiratory disturbances in Alzheimer’s disease: A focus on the contribution of sympathetic premotor neurons. Am Physiol Summit 39(S1) doi: 10.1152/physiol.2024.39.S1.2540 Objective: To characterize cardiorespiratory function in AD patients and then to determine the role of RVLM-C1 neurons in autonomic and sleep-disordered breathing in APP/PS1 double transgenic mice, and experimental
Janes T, Cardani S, Pagliardini S (2024) The progestinic drug, etonogestrel, rescues breathing in a rodent model of central chemoreflex impairment. Am Physiol Summit 39(S1) doi: 10.1152/physiol.2024.39.S1.978 Objective: Assessing the efficacy of etonogestrel as a treatment to congenital hypoventilation syndrome (CCHS). Summary: Etonogestrel can stimulate breathing and CO2-chemoreflexes and could be used to treat congenital
Kniffin A, Bangasser DA, Parikh V (2024) Septohippocampal cholinergic system at the intersection of stress and cognition: Current trends and translational implications. Eur J Neurosci 59(9):2155-2180. doi: 10.1111/ejn.15999 PMID: 37118907 Objective: Review current research highlighting the contributions of cholinergic septohippocampal pathway (SHP) in modulating memory encoding, consolidation, and retrieval. Summary: Authors presented evidence generated from
Stockdale JN (2024) Pollen-specific expression of ecori restriction endonuclease for bioconfinement in panicum virgatum l. Univ Tennessee Thesis. Objective: In this study, pollen-specific promoters controlling the expression of the EcoRI endonuclease, interrupted by a catalase intron, were evaluated for efficacy to produce sterile pollen. Summary: The TaPSG719, PvPS1, Osg6B, OsRTS, and Zm13 promoters were assessed
Ogata J, Shimada Y, Ohashi T, Kobayashi H (2024) Usefulness of antibody-drug conjugate as preconditioning for hematopoietic stem cell-targeted gene therapy in wild-type and Fabry disease mouse models. Mol Genet Metab 142(3):108494. doi: 10.1016/j.ymgme.2024.108494 PMID: 38820907 Objective: To develop a saporin/antibody-based conditioning for hematopoietic stem cell-targeted gene therapy for use in models of Fabry Disease.
Bohl JM, Hassan AR, Sharpe ZJ, Kola M, Ayub M, Pandey Y, Shehu A, Ichinose T (2024) Melanopsin ganglion cells in the mouse retina independently evoke pupillary light reflex. bioRxiv 2024.05.14.594181. doi: 10.1101/2024.05.14.594181 Objective: To examine the role of rod and cone photoreceptors in pupillary light reflex (PLR) by acutely ablating photoreceptors. Summary: Results demonstrate
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