Hovinga KE, Shimizu F, Wang R, Panagiotakos G, Van Der Heijden M, Moayedpardazi H, Correia AS, Soulet D, Major T, Menon J, Tabar V (2010) Inhibition of notch signaling in glioblastoma targets cancer stem cells via an endothelial cell intermediate. Stem Cells 28:1019-1029. doi: 10.1002/stem.429 PMID: 20506127 Summary: The antibody CD105 (1:1,000) was incubated with […]
Vincent BG, Young EF, Buntzman AS, Stevens R, Kepler TB, Tisch RM, Frelinger JA, Hess PR (2010) Toxin-coupled MHC class I tetramers can specifically ablate autoreactive CD8+ T cells and delay diabetes in nonobese diabetic mice. J Immunol 184(8):4196-4204. doi: 10.4049/jimmunol.0903931 Summary: MHC class I tetramers have been used to identify antigen-specific cells. In this
Klinkenberg I, Blokland A (2010) The validity of scopolamine as a pharmacological model for cognitive impairment: A review of animal behavioral studies. Neurosci Biobehav Rev 34(8):1307-1350. doi: 10.1016/j.neubiorev.2010.04.001 PMID: 20398692 Objective: To provide an overview is given of the effects of scopolamine on animal behavior. Summary: The most important and influential articles over the past
Marques-Lopes J, Pinho D, Albino-Teixeira A, Tavares I (2010) The hyperalgesic effects induced by the injection of angiotensin II into the caudal ventrolateral medulla are mediated by the pontine A(5) noradrenergic cell group. Brain Res 1325:41-52. doi: 10.1016/j.brainres.2010.02.043 Summary: Injection of angiotensin II into the caudal ventrolateral medulla (CVLM) has been shown to induce angiotensin
Vianna DM, Carrive P (2010) Cardiovascular and behavioural responses to conditioned fear and restraint are not affected by retrograde lesions of A5 and C1 bulbospinal neurons. Neuroscience 166:1210-1218. doi: 10.1016/j.neuroscience.2010.01.039 Summary: To investigate the role of A5 neurons in some forms of psychological stress the authors injected 22 or 44 ng of anti-DBH-SAP (Cat. #IT-03)
New Secondary Conjugates – Fab-ZAPs – Use Monovalent Secondary Antibodies Linked to Saporin For the past decade scientists have extensively used secondary conjugates (e.g. Mab-ZAP; Cat. #IT-04, and Hum-ZAP; Cat. #IT-22) to make their own targeted toxins for in vitro use. The ability to combine: 1) a primary antibody to a cell-surface marker with 2)
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Anti-6 His-SAP (Cat. #IT-52) Composed of an antibody to 6 His conjugated to saporin, this secondary conjugate should be used as a diagnostic tool for testing your primary, 6 His-tagged proteins for specific cell surface epitope binding and internalization. The 6 His tag is widely used because of its affinity to bind nickel or cobalt
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Anti-GFP-SAP (Cat. #IT-53) The green fluorescent protein (GFP) gene can be introduced and maintained in the genome through breeding, injection with a viral vector, or cell transformation. Whether your cells express a surface protein with a GFP-coded region, or you have a protein that targets specific cells and contains a GFP tag, Anti-GFP-ZAP can be
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Dailey MJ, Bartness TJ (2010) Arcuate nucleus destruction does not block food deprivation-induced increases in food foraging and hoarding. Brain Res 1323:94-108. doi: 10.1016/j.brainres.2010.01.078 Summary: While some aspects of food intake are understood, mechanisms controlling hoarding of food have not been identified. This work investigates the role of NPY in the arcuate nucleus (Arc) in
Gulino R, Perciavalle V, Gulisano M (2010) Expression of cell fate determinants and plastic changes after neurotoxic lesion of adult mice spinal cord by cholera toxin-B saporin. Eur J Neurosci 31(8):1423-1434. doi: 10.1111/j.1460-9568.2010.07170.x Summary: Sonic hedgehog, Notch-1, and Numb are proteins known to be involved in the function of stem cells. Understanding of how they