Targeting Tools: CTB-SAP

CTB-SAP (Cat. #IT-14) is a conjugate between the cell-binding component of cholera toxin (the B chain) and saporin. CTB binds to GM1 (monosialotetrahexosylganglioside), which is present on the surface of different neurons. It has been suggested to be involved in many problems (besides the most famous in the gut: cholera) of neuronal systems: Parkinson’s, motor neuron degeneration, spinal cord injury, and Alzheimer’s disease among others (see cover article).

The percent shift determined by flow cytometry staining with CTB-FITC was plotted against the log concentration of the EC50 in moles/L of CTB-SAP for six cell lines. The data show a distinct correlation between the number of CTB binding sites and the EC50 of CTB-SAP.