Targeting Tools: Top Twenty – Advanced Targeting Systems

Top Five Targeted Toxins

#1. 192-IgG-SAP (192-Saporin) (Cat. #IT-01) targets cells expressing rat p75NTR

#2. Anti-DBH-SAP (Cat. #IT-03) targets cells expressing rat dopamine beta-hydroxylase (DBH)

#3. mu p75-SAP (Cat. #IT-16) targets cells expressing mouse p75NTR

#4. IB4-SAP (Cat. #IT-10) targets cells expressing α-D-galactopyranoside residues

#5. Mac-1-SAP mouse/human (Cat. #IT-06) targets cells expressing mouse / human mac-1 (CD11b) receptor

TOP TARGETED TOXIN OF 2013

192-IgG-SAP (192-Saporin) (Cat. #IT-01) targets cells expressing rat p75NTR

Lesion with anti-DBH-SAP (DSAP) aimed at the PVN leads to dramatic loss of DBH-labeled innervation in the PVN, but also other parts of the hypothalamus (dorsomedial [DMH], lateral/perifornical [LH/PF], ventral tuberomammillary [TMV] portions), as well as the ventral tegmental area (VTA) and the ventrolateral periaqueductal grey (VLPAG) and dorsal raphe (DR). Telencephalic regions targeted by the locus coeruleus were not affected. Contributed by Lisa E. Goehler and Ronald P.A. Gaykema

Top Five Secondary Conjugates

#1. Mab-ZAP (Cat. #IT-04) Uses your primary mouse monoclonal antibody

#2. Streptavidin-ZAP (Cat. #IT-27) Uses your biotinylated material in order to evaluate the ability of the reagent to internalize upon binding to its receptor

#3. Anti-6 His-SAP (Cat. #IT-52) Uses your 6-His-tagged proteins over-expressed in cells

#4. Rab-ZAP (Cat. #IT-05) Uses your primary rabbit affinity-purified polyclonal IgG antibody to target and eliminate cells

#5. Fab-ZAP rat (Cat. #IT-55) Uses your primary rat monoclonal IgG antibody

TOP SECONDARY CONJUGATE OF 2013

Fab-ZAP human (Cat. #IT-51) Uses your primary human monoclonal IgG antibody to target

Top Five Antibodies

1. NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

2. NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

3. trkA Rabbit Polyclonal (Cat. #AB-N03)

4. NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

5. Angiotensin II receptor (AT-2r) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

TOP ANTIBODY OF 2013

NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

AB-N01AP. Immunofluorescent staining of NG6 cells, a clone of the NG108-15 fusion of a mouse neuroblastoma and rat glioblastoma. Cells were fixed with paraformaldehyde and blocked prior to staining with primary at 10 μg/ml followed by goat anti-rabbit-FITC secondary at 50 μg/ml and DAPI at 5 μg/ml for nuclear staining. Images were obtained using a 40x objective and a LeicaDM IL fluorescent microscope. NGFr staining is represented in green and nuclear staining is represented in blue.

Top Five in Publications

#1. 192-IgG-SAP (192-Saporin) (Cat. #IT-01) targets cells expressing rat p75NTR

#2. Anti-DBH-SAP (Cat. #IT-03) targets cells expressing rat dopamine beta-hydroxylase (DBH)

#3. SSP-SAP (Cat. #IT-11) targets cells expressing substance P (NK-1) receptor

#4. mu p75-SAP (Cat. #IT-16) targets cells expressing mouse p75NTR

#5. Streptavidin-ZAP (Cat. #IT-27) Uses your biotinylated material in order to evaluate the ability of the reagent to internalize upon binding to its receptor