The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally. The 6-His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state.
Anti-6-His-SAP is a chemical conjugate of the mouse monoclonal antibody to 6 His and the ribosome-inactivating protein, saporin. It specifically eliminates cells with extracellular expression of 6 His-tagged proteins and cells that recognize YOUR 6 His-tagged recombinant proteins.