Targeting Tools: Tag-Targeted Toxins

Tag-targeted toxins are new conjugates in the Targeted Toxins family. Antibodies specific for tags on expressed proteins are bound to the ribosome-inactivating protein, saporin (from the seeds of the plant, Saponaria officinalis). The conjugate binds to your tag that is expressed on the cell surface and then internalizes by antibody-mediated endocytosis. Saporin breaks away from the targeting agent, and inactivates the ribosomes which causes protein synthesis inhibition and, ultimately, cell death. Cells that do not have the tag are not affected.

Anti-6 His-SAP (Cat. #IT-52)
Specifically eliminates cells expressing your 6 His-tagged recombinant proteins on the cell surface.

Anti-GFP-SAP (Cat. #IT-53)
Specifically eliminates expressing your cells demonstrating extracellular expression of green fluorescent protein (GFP).

Anti-V5-SAP (Cat. #IT-58)
Specifically eliminates cells expressing your extracellular V5.

Anti-FLAG (M5)-SAP (Cat. #IT-59)
Specifically eliminates cells expressing your FLAG-tagged recombinant proteins on the cell surface.

Control for Tag-Targeted Toxins
Mouse IgG-SAP (Cat. #IT-18) is the same molecular weight, consists of similar, comparable materials and is synthesized with the same protocols as the tag-targeted toxins. The difference between targeted toxins and controls is the cell-specific targeting agents are replaced with “blanks,” antibodies or peptides that have no specificity, and no ability to target cells. Mouse IgG-SAP is the perfect control molecule to be used with these Tag-Targeted Toxins.

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